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首页> 外文期刊>Biochemistry >Steady-State Kinetic Investigation of Cytochrome P450cam:Interaction with Redox Partners and Reaction with Molecular Oxygen
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Steady-State Kinetic Investigation of Cytochrome P450cam:Interaction with Redox Partners and Reaction with Molecular Oxygen

机译：细胞色素P450cam的稳态动力学研究：与氧化还原伴侣的相互作用和与分子氧的反应

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摘要

Cytochrome P450cam (CYP101) is a prokaryotic monooxygenase that requires two proteins,putidaredoxin reductase (PdR) and putidaredoxin (Pdx),to supply electrons from NADH.This study addresses the mechanism by which electrons are transported from PdR to P450cam through Pdx and used to activate O_2 at the heme of P450cam.It is shown that k_(cat)/K_m(O_2) is independent of the PdR concentration and hyperbolically dependent on Pdx.The phenomenon of saturation of reaction rates with either P450cam or PdR at high rations of one enzyme to the other is investigated and shown to be consistent with a change in the rate limiting step.Either the reduction of Pdx by PdR (high P450) or the reduction of P450 by Pdx (high PdR) determines the rate.These data support a mechanism where Pdx acts as a shuttle for transport of electrons from PdR to P450cam,effectively ruling out the formation of a kinetically significant PdR/Pdx/P450cam complex.
机译：细胞色素P450cam（CYP101）是一种原核生物单加氧酶，需要两种蛋白质，即氧化还原蛋白还原酶（PdR）和氧化还原酶（Pdx）来提供来自NADH的电子。在P450cam的血红素上激活O_2，结果表明k_（cat）/ K_m（O_2）与PdR的浓度无关，并且与Pdx呈双曲线关系.P450cam或PdR在高比例的情况下反应速率饱和的现象研究表明，这种酶与另一种酶之间的变化与速率限制步骤的变化是一致的.PdR降低Pdx（高P450）或Pdx降低P450（高PdR）决定了速率。 Pdx充当将电子从PdR传输到P450cam的穿梭机构，有效地排除了动力学上重要的PdR / Pdx / P450cam复合物的形成。

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