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首页> 外文期刊>Biochemistry >Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing
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Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing

机译：腺苷天生就被认为是类似于天然RNA剪接的结构中的分支位点RNA核苷酸

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摘要

We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2',5'-branched RNA using a branch-site adenosine nucleophile and a Y-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly selected to use uridine as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing instead prefer a branch-site adenosine, although adenosine was never available during the selection itself. Our results support a chemical basis for nature's choice of the branch-site nucleotide, which is almost always adenosine in group II introns and the spliceosome.
机译：我们以前使用体外选择来鉴定7S11脱氧核酶，该酶​​使用分支位点腺苷亲核试剂和Y-三磷酸亲电试剂催化2'，5'-支链RNA的形成。一个悬而未决的问题是，在特定的选择实验期间，固有地优先使用分支位点腺苷还是偶然事件。在这里，我们发现新选择的脱氧核酶在结构上类似于自然RNA剪接，而是使用尿苷作为分支位点RNA核苷酸，而更喜欢使用分支位点腺苷，尽管在选择过程中腺苷从未获得过。我们的结果为自然选择分支位点核苷酸提供了化学基础，该分支位点核苷酸在II组内含子和剪接体中几乎总是腺苷。

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