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首页> 外文期刊>Biochemistry >Influence of Reduction of Heme a and Cu_A on the Oxidized Catalytic Center of Cytochrome c Oxidase:Insight from Organic Solvents
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Influence of Reduction of Heme a and Cu_A on the Oxidized Catalytic Center of Cytochrome c Oxidase:Insight from Organic Solvents

机译：血红素a和Cu_A的还原对细胞色素c氧化酶氧化催化中心的影响：有机溶剂的认识

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摘要

Purified bovine heart cytochrome c oxidase (CcO) has been extracted from aqueous solution into hexane in the presence of phospholipids and calcium ions.In extracts,CcO is in the so-called "slow" form and probably situated in reverse micelles.At low watenphospholipid molar ratios,electron transfer from reduced heme a and Cu_A to the catalytic center is inhibited and both heme a_3 and Cu_B remain in the oxidized state.The rate of binding of cyanide to heme a_3 in this oxidized catalytic center is,however,dependent on the redox state of heme a and Cu_A.When heme a and Cu_A are reduced,the rate is increased 20-fold compared to the rate when these two centers are oxidized.The enhanced rate of binding of cyanide to heme a_3 is explained by the destabilization of an intrinsic ligand,located at the catalytic site,that is triggered by the reduction of heme a and Cu_A.
机译：在存在磷脂和钙离子的情况下，已从水溶液中将纯化的牛心脏细胞色素c氧化酶（CcO）提取到己烷中。在提取物中，CcO呈所谓的“慢”形式，可能位于反胶束中。摩尔比，抑制了从还原血红素a和Cu_A到催化中心的电子转移，并且血红素a_3和Cu_B都保持氧化态。但是，在该氧化催化中心中氰化物与血红素a_3的结合速率取决于血红素a和Cu_A的氧化还原状态。当血红素a和Cu_A减少时，该速率比这两个中心被氧化时的速率增加20倍。氰化物与血红素a_3的结合速率提高是由于一种固有的配体，位于催化位点，由血红素a和Cu_A的还原触发。

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