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首页> 外文期刊>Biochemistry >A Thermodynamic Comparison of HPr Proteins from Extremophilic Organisms
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A Thermodynamic Comparison of HPr Proteins from Extremophilic Organisms

机译：来自极端微生物的HPr蛋白的热力学比较。

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摘要

A thermodynamic stability study of five histidine-containing phosphocarrier protein (HPr) homologues derived from organisms inhabiting diverse environments is described.These HPr homologues are from Bacillus subtilis (Bs),Streptococcus thermophilus (St),Bacillus staerothermophilus (Bst),Bacillus halodurans (Bh),and Oceanobacillus iheyensis (Oi).Analyses of solvent and thermal denaturation experiments provide the cardinal thermodynamic parameters,like DELTA G,DELTA H,DELTA S,T_m,and DELTA C_P,that characterize the conformational stability for each homologue.The homologue from Bacillus staerothermophilus (BstHPr) was established as the most thermostable homologue and also the homologue with highest DELTA G at all temperatures.A good correlation between habitat temperature of the organism and thermal stability of the protein is also seen.Stability curves (DELTA G vs T) for every homologue are also reported;these reveal very similar DELTA C_P and temperature of maximum stability (Ts) values for all HPr homologues.Stability curves show that the higher thermal stability of some homologues is not a result of change in curvature of the curve or a shift to higher temperature,but rather a displacement of the stability curves to higher DELTA G values.Stability curves also allowed estimation of DELTA G at habitat temperature of the organisms,and we find good agreement between homologues.Electrostatic contributions to stability of each homologue were investigated by measuring stability as a function of varying pH and NaCl concentration,and our results suggest that most HPr homologues share similar electrostatic contributions to stability.
机译：对五个来自不同环境的生物的含组氨酸的磷酸载体蛋白（HPr）同源物的热力学稳定性进行了研究，这些同源物分别来自枯草芽孢杆菌（Bs），嗜热链球菌（St），嗜热芽孢杆菌（Bst），嗜碱性芽孢杆菌（Bacillus halodurans）（溶剂和热变性实验的分析提供了主要的热力学参数，例如DELTA G，DELTA H，DELTA S，T_m和DELTA C_P，它们表征了每个同源物的构象稳定性。嗜热脂肪芽孢杆菌（BstHPr）的建立是在所有温度下最热稳定的同系物，也是DELTA G最高的同系物。生物体的栖息地温度与蛋白质的热稳定性之间也有很好的相关性。也记录了每个同系物的T）;这些揭示非常相似的DELTA C_P和最大稳定性（Ts）值的温度稳定性曲线表明，某些同源物的较高热稳定性不是曲线曲率变化或温度升高的结果，而是稳定性曲线向较高DELTA G值位移的结果。曲线还可以估算出生物体栖息地温度下的DELTA G，我们发现同系物之间有很好的一致性。通过测量稳定性随pH和NaCl浓度的变化来研究静电对每种同系物稳定性的贡献，我们的结果表明大多数HPr同源物对稳定性具有相似的静电贡献。

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