Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures

K. Danielle Kelley; Lorenzo Q. Olive; Arina Hadziselimovic; Charles R. Sanders

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Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures

机译：看看是否是时候在大肠杆菌培养物中诱导蛋白质表达了

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It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.

机译：结果表明，由于细菌产生的偶氮还原酶对时间和细胞密度的依赖性漂白作用，甲基红可用作指示剂染料，可在大肠杆菌培养物中改变颜色。对于用于表达重组蛋白的细胞培养物，可以利用这种现象来提供简单的视觉提示，即细胞培养物已经达到适当的生长期，以添加诱导蛋白表达的试剂，例如异丙基硫代半乳糖苷。

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《Biochemistry》 |2010年第26期|共3页
作者
K. Danielle Kelley; Lorenzo Q. Olive; Arina Hadziselimovic; Charles R. Sanders;
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原文格式 PDF
正文语种 eng
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关键词
Time; Protein; Expression;

机译：时间;蛋白质;表达;

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1. Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures [J] . K. Danielle Kelley Lorenzo Q. Olive Arina Hadziselimovic and Charles R. Sanders Biochemistry . 2010,第26期

机译：看看是否是时候在大肠杆菌培养物中诱导蛋白质表达了
2. Fractional factorial approach combining 4 Escherichia coli strains, 3 culture media, 3 expression temperatures and 5 N-terminal fusion tags for screening the soluble expression of recombinant proteins [J] . Noguère C., Larsson A.M., Guyot J.-C., Protein Expression and Purification . 2012,第2期

机译：分数阶乘方法结合4种大肠杆菌菌株，3种培养基，3种表达温度和5种N末端融合标签来筛选重组蛋白的可溶性表达
3. Protein d, an iron‐transport protein induced by filtration of cultures of Escherichia coli [J] . Boyd A., Holland I.B. FEBS Letters . 1977,第1期

机译：蛋白质d，一种通过过滤大肠杆菌培养物诱导的铁转运蛋白
4. Heterologous proteins expression in Escherichia coli: investigation of the effect of codon usage and expression host optimization [C] . Hasan Mirzahoseini, Samaneh Mafakheri, Somayeh Enayati, International Conference on Environmental, Industrial and Applied Microbiology . 2009

机译：Escherichia Coli中的异源蛋白表达：对密码子使用和表达宿主优化的影响的研究
5. Proteomic profiling and label-free quantification of bovine milk protein expression during experimentally induced Escherichia coli mastitis. [D] . Boehmer, Jamie Layne. 2009

机译：蛋白质组学分析和实验诱导的大肠杆菌乳腺炎期间牛乳蛋白表达的无标记定量。
6. Look and See if it is Time to Induce Protein Expression in Eschericia coli Cultures [O] . K. Danielle Kelley, Lorenzo Q. Olive, Arina Hadziselimovic, -1

机译：看看看看现在是时候引起大肠杆菌中培养蛋白表达
7. Protein d, an iron-transport protein induced by filtration of cultures of Escherichia coli [O] . Boyd A., Holland I.B. 1977

机译：蛋白d，通过过滤大肠杆菌培养物诱导的铁转运蛋白

Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures

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