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首页> 外文期刊>Biochemistry >The TatBC Complex of the Tat Protein Translocase in Escherichia coli and Its Transition to the Substrate-Bound TatABC Complex
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The TatBC Complex of the Tat Protein Translocase in Escherichia coli and Its Transition to the Substrate-Bound TatABC Complex

机译：TatBC复杂的TatBC在大肠杆菌中的蛋白质及其向底物结合的TatABC复合物的转变

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摘要

The twin-arginine translocation (Tat) system serves to transport folded proteins across membranes of prokaryotes and plant plastids. In Escherichia coli, a complex consisting of multiple copies of TatB and TatC initiates the transport by binding the signal peptides of the Tat substrates. Using blue-native polyacrylamide gel electrophoresis, bands of TatBC-containing complexes can be detected at molecular masses of 440 and 580 kDa. We systematically analyzed the formation of Tat complexes with TatB or TatC variants that carried point mutations at selected positions. Several mutations resulted in specific disassembly patterns and alterations in the 440 kDa:580 kDa complex ratios. The 440 kDa complex contains only TatBC, whereas the 580 kDa complex consists of TatABC. Substrate binding results in a TatBC?Tat substrate complex at ～500 kDa and a TatABC?Tat substrate complex at ～600 kDa. Only the ～600 kDa complex was detected with nonrecombinant substrate levels and thus could be the physiologically most relevant species. The results suggest that some TatA is usually associated with TatBC, regardless of substrate binding.
机译：双精氨酸易位（Tat）系统用于将折叠的蛋白质跨原核生物和植物质体的膜运输。在大肠杆菌中，由多个拷贝的TatB和TatC组成的复合物通过结合Tat底物的信号肽来启动转运。使用蓝色天然聚丙烯酰胺凝胶电泳，可以检测到分子量为440和580 kDa的TatBC配合物的条带。我们系统地分析了带有TatB或TatC变体的Tat复合物的形成，这些变体在选定位置携带点突变。几个突变导致特定的拆卸模式和440 kDa：580 kDa复杂比率的变化。 440 kDa复合体仅包含TatBC，而580 kDa复合体则由TatABC组成。底物结合导致〜500 kDa的TatBC？Tat底物复合物和〜600 kDa的TatABC？Tat底物复合物。仅检测到〜600 kDa复合物，底物水平没有重组，因此可能是生理上最相关的物种。结果表明，某些TatA通常与TatBC相关，而与底物结合无关。

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