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首页> 外文期刊>Biomaterials >The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia.
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The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia.

机译：hFOB 1.19成骨细胞对表面修饰的钛和氧化锆的基因表达和表型反应。

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摘要

The osteoblastic cell-line hFOB 1.19 with the potential to proliferate and differentiate revealed that cellular differentiation is not affected by material and roughness on newly developed zirconia implant materials. Materials under investigation were surfaces machined titanium (Ti-m), modified titanium (TiUnite, machined zirconia (TZP-A-m), modified zirconia (ZiUnitemachined alumina-toughened zirconia (ATZ-m) and modified alumina-toughened zirconia (ATZ-mod). After surface description by scanning electron microscopy (SEM) and atomic force microscopy (AFM), cellular proliferation (EZ4U, Casy1) and differentiation were examined after days 1, 3, 7, 14, 21, and 28. Osteogenic differentiation was visualized by alkaline phosphatase staining, mineralization assay (alizarin red) and by expression analysis (RT-PCR) of bone- and extracellular matrix-related genes. Proliferation on rough surfaces was reduced on both titanium and zirconia. Cell-attachment and cytoskeleton organization documented by confocal laserscanning microscopy (CLSM) elucidated attenuated cell attachment within the first 4h to be the reason for impaired proliferation. A specific up-regulation of m-RNAs in an early event (RUNX2, NELL-1, RUNX3, and BMP7) and a late event (Integrin B3) could be observed on TiUnite and ZiUnite. For titanium an up-regulation of IBSP and Integrin B1 could be described at day 21. In total, differentiation was neither affected by material nor by roughness.
机译：具有增殖和分化潜能的成骨细胞系hFOB 1.19表明，新开发的氧化锆植入物材料的细胞分化不受材料和粗糙度的影响。所研究的材料为表面机加工的钛（Ti-m），改性的钛（TiUnite，机加工的氧化锆（TZP-Am），改性的氧化锆（ZiUnite机加工的氧化铝增韧氧化锆（ATZ-m）和改性的氧化铝增韧的氧化锆（ATZ-mod））在通过扫描电子显微镜（SEM）和原子力显微镜（AFM）进行表面描述后，在第1、3、7、14、21和28天后检查了细胞增殖（EZ4U，Casy1）和分化。碱性磷酸酶染色，矿化分析（茜素红）和骨与细胞外基质相关基因的表达分析（RT-PCR）;钛和氧化锆在粗糙表面的增殖均减少;共聚焦记录的细胞附着和细胞骨架组织激光扫描显微镜（CLSM）阐明了最初4h内细胞粘附减弱是增殖受损的原因。早期事件中m-RNA的特定上调（RUNX2，NELL-1，R在TiUnite和ZiUnite上可以观察到UNX3和BMP7）以及一个晚期事件（整合素B3）。对于钛，可以在第21天描述IBSP和整联蛋白B1的上调。总的来说，差异既不受材料的影响，也不受粗糙度的影响。

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