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首页> 外文期刊>Bioorganic and Medicinal Chemistry Letters >A chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents.
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A chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents.

机译：化学修饰的脂肪酶制剂，即使在高极性有机溶剂中也能催化酯交换反应。

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摘要

Acylation of Pseudomonas cepacia lipase with Pyromellitic dianhydride to modify 72% of total amino groups was carried out. Different organic solvents were screened for precipitation of modified lipase. It was found that 1,2-dimethoxyethane was the best precipitant which precipitated 97% protein and complete activity. PCMC (protein coated microcrystals), CLPCMC (crosslinked protein coated microcrystals), EPROS (enzyme precipitated and rinsed with organic solvents) and pH tuned preparations of modified and unmodified lipase were prepared and used for carrying out transesterification reaction with n-octane and dimethyl formamide (DMF) as reaction medium. In n-octane, among all the preparations, CLPCMC of modified lipase gave highest rate (1970 nmol min(-1)mg(-1)) as compared to unmodified pH tuned lipase (128 nmol min(-1) mg(-1)). In DMF, with both 1% (v/v) and 5% (v/v) water content, CLPCMC showed highest initial rate of 0.72 and 7.2 nmol min(-1) mg(-1), respectively. Unmodified pH tuned lipase showed no activity at all in DMF with both 1% and 5% (v/v) water content.
机译：用均苯四甲酸二酐酰化洋葱假单胞菌脂肪酶，以修饰72％的总氨基。筛选不同的有机溶剂以沉淀修饰的脂肪酶。发现1,2-二甲氧基乙烷是最好的沉淀剂，沉淀出97％的蛋白质并具有完整的活性。制备了PCMC（蛋白包被的微晶），CLPCMC（交联的蛋白包被的微晶），EPROS（酶沉淀并用有机溶剂冲洗）和经pH调节的改性和未改性脂肪酶制剂，并用于与正辛烷和二甲基甲酰胺进行酯交换反应（DMF）作为反应介质。在所有制剂中，正辛烷中的修饰脂肪酶的CLPCMC与未修饰的pH值调节的脂肪酶（128 nmol min（-1）mg（-1）相比，产生率最高（1970 nmol min（-1）mg（-1）） ））。在DMF中，水含量分别为1％（v / v）和5％（v / v）时，CLPCMC的最高初始比率分别为0.72和7.2 nmol min（-1）mg（-1）。未经修饰的pH调节的脂肪酶在1％和5％（v / v）的水含量下在DMF中完全没有活性。

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