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首页> 外文期刊>Biochemical and Biophysical Research Communications >Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen
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Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen

机译：含牛血清白蛋白的磁化填充剂对公猪精液长期保存过程中精子氧化应激的抗氧化作用

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摘要

Magnetized water is defined as water that has passed through a magnet and shows increased permeability into cells and electron-donating characteristics. These attributes can protect against membrane damage and remove reactive oxygen species (ROS) in mammalian cells. We explored the effects of improved magnetized semen extenders containing bovine serum albumin (BSA) as antioxidants on apoptosis in boar sperm. Ejaculated semen was diluted in magnetized extender (OG and 6000G) with or without BSA COG + BSA and 6000G + BSA), and sperm were analyzed based on viability, acrosome reaction, and H2O2 level of live sperm using flow cytometry. Sperm were then preserved for 11 days at 18 degrees C. We found that viability was significantly higher in 6000G + BSA than under the other treatments (P < 0.05). The acrosome reaction was significantly lower in the 6000G + BSA group compared with the other treatments (P < 0.05). Live sperm with high intracellular H2O2 level were significantly lower in the 6000G + BSA group than under other treatments (P < 0.05). Based on our results, magnetized extenders have antioxidative effects on the liquid preservation of boar sperm. (C) 2015 Elsevier Inc. All rights reserved.
机译：磁化水定义为已通过磁体并显示出进入细胞的渗透性和给电子特性的水。这些属性可以防止膜损伤并去除哺乳动物细胞中的活性氧（ROS）。我们探讨了改良的含牛血清白蛋白（BSA）抗氧化剂的精液精子补充剂对公猪精子凋亡的影响。射精后的精液在有或没有BSA COG + BSA和6000G + BSA的磁化增量剂（OG和6000G）中稀释，并使用流式细胞仪根据活力，顶体反应和活精中H2O2的水平分析精子。然后将精子在18摄氏度下保存11天。我们发现，在6000G + BSA中，活力显着高于其他治疗（P <0.05）。与其他治疗相比，6000G + BSA组的顶体反应显着降低（P <0.05）。 6000G + BSA组的细胞内H2O2水平较高的活精子明显低于其他治疗组（P <0.05）。根据我们的结果，磁化增量剂对公猪精液的保存具有抗氧化作用。 （C）2015 Elsevier Inc.保留所有权利。

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