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首页> 外文期刊>Biochemical and Biophysical Research Communications >Transcription outcome of promoters enriched in histone variant H3.3 defined by positioning of H3.3 and local chromatin marks
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Transcription outcome of promoters enriched in histone variant H3.3 defined by positioning of H3.3 and local chromatin marks

机译：通过定位H3.3和局部染色质标记而定义的富含组蛋白变体H3.3的启动子的转录结果

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摘要

Replication-independent histone variant H3.3 is incorporated into distinct genomic regions including promoters. However topology of promoter-associated H3.3 in relation to chromatin modifications and transcriptional outcome is not known, providing no insight on any distinction between H3.3-containing active and inactive promoters. Here, we report algorithms providing information on gene expression status as a function of density and position of multiple chromatin marks on promoters. We identify a relationship between promoter enrichment in epitope-tagged H3.3 or its canonical isoform H3.2 and corresponding transcriptional outcomes, as a function of sub-promoter positioning of DNA methylation and H3K4me3, H3K9me3 and H3K27me3. We identify a low-frequency configuration of H3.3 and H3K9me3 co-occupancy associated with transcriptional activity, while H3.3 and H3K27me3 promoters are invariably inactive. We unveil H3.3 and DNA methylated promoters whose transcription outcome depends on H3.3 sub-promoter positioning. Our results indicate how spatially restricted positioning of H3.3 may add another layer of transcription regulation. (C) 2015 Elsevier Inc. All rights reserved.
机译：不依赖复制的组蛋白变体H3.3被掺入不同的基因组区域，包括启动子。然而，与染色质修饰和转录结果相关的与启动子相关的H3.3的拓扑结构是未知的，因此无法提供关于含H3.3的活性和非活性启动子之间任何区别的见解。在这里，我们报告的算法提供了有关基因表达状态的信息，这些信息是启动子上多个染色质标记的密度和位置的函数。我们确定的启动子富集在抗原决定簇标记的H3.3或其规范的同种型H3.2和相应的转录结果之间的关系，作为DNA甲基化和H3K4me3，H3K9me3和H3K27me3的子启动子定位的函数。我们发现与转录活性相关的H3.3和H3K9me3共同占据的低频配置，而H3.3和H3K27me3启动子始终是无活性的。我们揭示了H3.3和DNA甲基化的启动子，其转录结果取决于H3.3子启动子的定位。我们的结果表明，H3.3的空间受限定位可能会增加另一层转录调控。 （C）2015 Elsevier Inc.保留所有权利。

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