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首页> 外文期刊>Brain research >Na+/Ca2+ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia.
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Na+/Ca2+ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia.

机译：缺血后，Na + / Ca2 +交换子1在锥体细胞中改变，并在沙鼠海马CA1区的星形胶质细胞中表达。

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摘要

Alterations of immunoreactivity and protein contents of Na(+)/Ca(2+) exchanger 1 (NCX1) were observed in the gerbil hippocampus proper after 5 min of transient forebrain ischemia. NCX1 immunoreactivity was significantly changed in the hippocampal CA1 region, but not in the CA2/3 region after ischemia/reperfusion. In the sham-operated group, NCX1 immunoreactivity was mainly detected in CA1 pyramidal cells. However, 30 min after ischemia/reperfusion, NCX1 immunoreactivity was significantly decreased and then increased at 1 day after ischemia. At this time, NCX1 immunoreactivity in CA1 pyramidal cells was similar to that of the sham-operated group. At 3 days after ischemia, NCX1 immunoreactivity was significantly reduced in the CA1 region compared to that of the sham-operated group and NCX1 immunoreactivity was significantly increased again 4 days after ischemia. Thereafter, NCX1 immunoreactivity was decreased time-dependently in ischemia groups. Between 15 min and 6 h post-ischemia, NCX1 immunoreactivitywas expressed in astrocytes in the strata oriens and radiatum of the CA1 region. From 3 days post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum. Ischemia-induced changes in NCX1 protein contents in the hippocampus proper concurred with immunohistochemical data post-ischemia. Our results suggest that changes in NCX1 in CA1 pyramidal cells and astrocytes after ischemia are associated with intracellular Na(+) concentrations and that NCX1 may induce an intracellular calcium overload, which may be related to neuronal death.
机译：Na（+）/ Ca（2+）交换子1（NCX1）的免疫反应性和蛋白质含量的变化在短暂的前脑缺血5分钟后在沙土鼠海马中观察到。缺血/再灌注后，海马CA1区的NCX1免疫反应性发生了显着变化，但CA2 / 3区的NCX1免疫反应却没有改变。在假手术组中，主要在CA1锥体细胞中检测到NCX1免疫反应性。然而，在缺血/再灌注后30分钟，NCX1免疫反应性显着降低，然后在缺血后1天增加。此时，CA1锥体细胞中的NCX1免疫反应性与假手术组相似。与假手术组相比，缺血后3天，CA1区的NCX1免疫反应性显着降低，缺血4天后NCX1免疫反应性又显着提高。此后，缺血组NCX1免疫反应性呈时间依赖性降低。在缺血后15分钟至6小时之间，NCX1免疫反应性在CA1区域的原始层和放射状的星形胶质细胞中表达。从缺血后3天起，NCX1免疫反应性在层状层和放射状层的星形胶质细胞中表达。缺血后海马中缺血诱导的NCX1蛋白含量的变化与免疫组织化学数据一致。我们的结果表明，缺血后CA1锥体细胞和星形胶质细胞中NCX1的变化与细胞内Na（+）浓度有关，并且NCX1可能引起细胞内钙超载，这可能与神经元死亡有关。

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