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首页> 外文期刊>Brain research >Detection and quantitative analysis of human herpesvirus in pilocytic astrocytoma.
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Detection and quantitative analysis of human herpesvirus in pilocytic astrocytoma.

机译：疱疹性星形细胞瘤中人类疱疹病毒的检测和定量分析。

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We investigated the hypothetical role of human herpesviruses (HHVs) in tumour formation of the cerebellum. Thirty-five samples of pilocytic astrocytoma and 10 control samples of cerebellum from patients who died of unrelated diseases were examined. Presence of the 8 known HHVs was first studied using specific real-time quantitative Polymerase Chain Reaction (qPCR) targeting viral DNA polymerase. HHV's DNA polymerase was found present in 20 samples (7 controls, 13 astrocytomas) and was absent in 25 samples (3 controls, 22 astrocytomas). DNA polymerase of Epstein-Barr Virus (EBV) was present in 16 samples, 7/10 controls (70%) and 9/35 astrocytomas (26%). HHV-1 and Varicella-Zoster virus were detected only twice and HHV-2, Cytomegalovirus, HHV-7 and HHV-8, only once. HHV-6 was not detected. In all cases, the gene copy numbers of DNA polymerase were low (<100/100 ng DNA). A second approach was to search for novel HHVs, using consensus-degenerated hybrid oligonucleotide primers (CODEHOP) PCR: no sequence indicative of a new HHV was detected. In summary, EBV was the most frequent HHV detected in pilocytic astrocytoma, but at very low levels. According to the actually accepted threshold the results suggest that EBV cannot be considered responsible for tumorigenesis of pilocytic astrocytoma.
机译：我们调查了人类疱疹病毒（HHVs）在小脑肿瘤形成中的假设作用。死于无关疾病的患者共检查了35份细胞性星形细胞瘤样本和10份小脑对照样本。首先使用针对病毒DNA聚合酶的实时定量聚合酶链反应（qPCR）研究了8种已知HHV的存在。发现HHV的DNA聚合酶存在于20个样品（7个对照，13个星形细胞瘤）中，而25个样品中不存在（3个对照，22个星形细胞瘤）。爱泼斯坦-巴尔病毒（EBV）的DNA聚合酶存在于16个样品中，其中7/10个对照（70％）和9/35个星形细胞瘤（26％）存在。仅检测到两次HHV-1和水痘带状疱疹病毒，而仅一次检测到HHV-2，巨细胞病毒，HHV-7和HHV-8。未检测到HHV-6。在所有情况下，DNA聚合酶的基因拷贝数都很低（<100/100 ng DNA）。第二种方法是使用共有简并的杂交寡核苷酸引物（CODEHOP）PCR搜索新型HHV：未检测到指示新HHV的序列。总之，EBV是毛细胞星形细胞瘤中最常见的HHV，但水平很低。根据实际接受的阈值，结果提示不能将EBV视为造成细胞性星形细胞瘤的肿瘤发生原因。

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