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首页> 外文期刊>Coral reefs: journal of the International Society for Reef Studies >An alternative to ITS, a hypervariable, single-copy nuclear intron in corals, and its use in detecting cryptic species within the octocoral genus Carijoa
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An alternative to ITS, a hypervariable, single-copy nuclear intron in corals, and its use in detecting cryptic species within the octocoral genus Carijoa

机译：ITS的替代品，珊瑚中的高变单拷贝核内含子，其用于检测八叶珊瑚属Carijoa中的隐性物种

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摘要

Here we report a highly variable nuclear marker that can be used for both soft and stony corals. Primers that amplify a similar to 177 bp fragment from the nuclear gene encoding the 54 kDa subunit of the signal recognition particle (SRP54) were developed for the octocoral genus Carijoa. Cloning results from 141 individuals suggest that this hypervariable nuclear locus is a single-copy gene. Sequencing revealed a potential cryptic species previously thought to be Carijoa riisei. Results from an Analysis of Molecular Variance (AMOVA) based on mitochondrial DNA (mtDNA) explained < 10% of the variation between Atlantic and Pacific samples of C. riisei (F-st = 0.47), whereas the same comparison with SRP54 explained > 33% of the variation (F-st = 0.54). Using previously reported degenerate primers for SRP54, high levels of sequence variation were found at this locus across both scleractinian and octocorals. For example, pairwise sequence divergence within octocorals was similar to 8-13 times greater with SRP54 than with mtDNA, and, up to 2.8% pairwise sequence divergence was found in SRP54 among individuals of Pocillopora whereas no variation at all was found in mtDNA markers. This case study with the octocoral C. riisei shows that variation in SRP54 appears sufficient to address questions of phylogeography as well as systematics of closely related species.
机译：在这里，我们报告了可用于软珊瑚和石珊瑚的高度可变的核标记。针对八叶珊瑚属Carijoa，开发了从编码信号识别颗粒（SRP54）54 kDa亚基的核基因扩增出类似于177 bp片段的引物。 141个个体的克隆结果表明，该高变核基因座是单拷贝基因。测序揭示了以前认为是Carijoa riisei的潜在隐性物种。基于线粒体DNA（mtDNA）的分子变异分析（AMOVA）的结果解释了 33变化的百分比（F-st = 0.54）。使用先前报道的SRP54简并引物，在该菌落和socractinian和octocorals中都发现了高水平的序列变异。例如，八齿虫内的成对序列差异比用mtDNA的SRP54大8-13倍，并且在Pocillopora个体中，在SRP54中发现高达2.8％的成对序列差异，而在mtDNA标记中根本没有发现差异。此案例研究以八齿丽珠菌为例，表明SRP54的变异似乎足以解决系统地理学问题以及紧密相关物种的系统问题。

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