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首页> 外文期刊>Chemistry: A European journal >Covalent microcontact printing of proteins for cell patterning
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Covalent microcontact printing of proteins for cell patterning

机译：蛋白质的共价微接触印刷，用于细胞模式

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摘要

We describe a straightforward approach to the covalent immobilization of cytophilic proteins by micro-contact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.
机译：我们描述了一种通过微接触印刷将亲细胞蛋白共价固定的简单方法，该方法可用于在底物上图案化细胞。通过使用亚胺化学方法，将嗜细胞性蛋白以微模式印刷在反应性自组装单层膜上。通过氨基封端的单层与对苯二醛之间的反应获得了玻璃或金上的醛封端的单层。醛单层被用作底物，通过使用氧化的聚二甲基硅氧烷（PDMS）印章直接微接触印刷生物工程化的胶原蛋白样蛋白质。将蛋白质固定在粘性“岛”上后，其余区域被氨基聚乙二醇封闭，形成了一层抗细胞粘附的层。将人恶性癌细胞（HeLa）接种并孵育到带图案的底物上。发现这些细胞粘附并选择性地扩散在蛋白质岛上，并避开了聚乙二醇（PEG）区。这些发现说明了微接触印刷作为将蛋白质定位在表面的一种方法的重要性，并证明了受控表面化学作用指导细胞粘附的范围。

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