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首页> 外文期刊>Chemistry: A European journal >Characterization of G-quadruplex/hemin peroxidase: Substrate specificity and inactivation kinetics
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Characterization of G-quadruplex/hemin peroxidase: Substrate specificity and inactivation kinetics

机译：G-四链体/血红素过氧化物酶的表征：底物特异性和失活动力学

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摘要

Recently, G-quadruplex/hemin (G4/hemin) complexes have been found to exhibit peroxidase activity, and this feature has been extensively exploited for colorimetric detection of various targets. To further understand and characterize this important DNAzyme, its substrate specificity, inactivation mechanism, and kinetics have been examined by comparison with horseradish peroxidase (HRP). G4/hemin DNAzyme exhibits broader substrate specificity and much higher inactivation rate than HRP because of the exposure of the catalytic hemin center. The inactivation of G4/hemin DNAzyme is mainly attributed to the degradation of hemin by H_2O_2 rather than the destruction of G4. Both the inactivation rate and catalytic oxidation rate of G4/hemin DNAzyme depend on the concentration of H_2O_2, which suggests that active intermediates formed by G4/hemin and H_2O_2 are the branch point of catalysis and inactivation. Reducing substrates greatly inhibit the inactivation of G4/hemin DNAzyme by rapidly reacting with the active intermediates. A possible catalytic and inactivation process of G4/hemin has been proposed. These results imply a potential cause for the hemin-mediated cellular injury and provide insightful information for the future application of G4/hemin DNA-zyme.
机译：最近，已发现G-四链体/ hemin（G4 / hemin）复合物表现出过氧化物酶活性，并且该特征已被广泛用于各种目标的比色检测。为了进一步理解和表征这种重要的DNA酶，已通过与辣根过氧化物酶（HRP）的比较检查了其底物特异性，失活机制和动力学。与HRP相比，G4 / hemin DNAzyme具有更宽的底物特异性和更高的灭活速率，因为它具有催化性的hemin中心。 G4 / hemin DNAzyme的失活主要归因于H_2O_2对Hemin的降解，而不是G4的破坏。 G4 / hemin DNAzyme的失活速率和催化氧化速率均取决于H_2O_2的浓度，这表明由G4 / hemin和H_2O_2形成的活性中间体是催化和失活的分支点。还原性底物通过与活性中间体快速反应，极大地抑制了G4 /血红素DNAzyme的失活。已经提出了可能的G4 /血红素催化和失活过程。这些结果暗示了血红素介导的细胞损伤的潜在原因，并为G4 /血红素DNA酶的未来应用提供了有见地的信息。

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