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首页> 外文期刊>Chemistry: A European journal >Nucleic acid driven DNA machineries synthesizing Mg~(2+)-dependent DNAzymes: An interplay between DNA sensing and logic-gate operations
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Nucleic acid driven DNA machineries synthesizing Mg~(2+)-dependent DNAzymes: An interplay between DNA sensing and logic-gate operations

机译：核酸驱动的DNA机械合成Mg〜（2+）依赖性DNA酶：DNA感测和逻辑门操作之间的相互作用

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摘要

Polymeraseicking enzymes and nucleic-acid scaffolds are implemented as DNA machines for the development of amplified DNA-detection schemes, and for the design of logic gates. The analyte nucleic acid target acts, also, as input for the logic gates. In the presence of two DNA targets, acting as inputs, and appropriate DNA scaffolds, the polymerase-induced replication of the scaffolds, followed by the nicking of the replication products, are activated, leading to the autonomous synthesis of the Mg~(2+)-dependent DNAzyme or the Mg~(2+)-dependent DNAzyme subunits. These biocatalysts cleave a fluorophore/quencher-functionalized nucleic-acid substrate, thus providing fluorescence signals for the sensing events or outputs for the logic gates. The systems are used to develop OR, AND, and Controlled-AND gates, and the DNA-analyte targets represent two nucleic acid sequences of the smallpox viral genome. Open the gate! Nucleic acid analytes (inputs) trigger polymerizationicking DNA machines that synthesize the Mg~(2+)- dependent DNAzymes. The systems allow the amplified detection of the analytes and the activation of the OR, AND, and controlled-AND logic gates by using fluorescence as output signal (see scheme).
机译：聚合酶/切口酶和核酸支架被用作DNA机器，用于开发扩增的DNA检测方案和设计逻辑门。被分析物核酸靶还充当逻辑门的输入。在存在两个作为输入的DNA靶标以及适当的DNA支架的情况下，激活了聚合酶诱导的支架复制，然后复制产物形成切口，从而导致Mg〜（2+依赖的DNAzyme或Mg〜（2+）依赖的DNAzyme亚基。这些生物催化剂裂解了荧光团/猝灭剂功能化的核酸底物，从而为检测事件提供荧光信号或为逻辑门提供输出。该系统用于开发“或”，“与”和“受控与”门，DNA分析物靶标代表天花病毒基因组的两个核酸序列。开门！核酸分析物（输入）触发聚合/刻痕DNA机器，该机器合成Mg〜（2+）依赖性DNA酶。该系统通过使用荧光作为输出信号，可以对分析物进行放大检测，并激活“或”，“与”和“受控与”逻辑门（请参见方案）。

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