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首页> 外文期刊>Chemistry: A European journal >Efficient DNA condensation induced by ruthenium(II) complexes of a bipyridine-functionalized molecular clip ligand
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Efficient DNA condensation induced by ruthenium(II) complexes of a bipyridine-functionalized molecular clip ligand

机译：联吡啶官能化分子夹子配体的钌（II）配合物诱导的高效DNA缩合

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摘要

Complexes of the type [Ru(bxbg)_2(N-N)]~(2+), where N-N denotes 2,2′-bipyridine (bpy) (1), 1,10-phenanthroline (phen) (2), dipyrido[3,2-d:2′,3-f] quinoxaline (dpq) (3), and dipyrido[3,2-a:2′, 3′-c]phenazine (dppz) (4), incorporating bis(o-xylene)bipyridine- glycoluril (bxbg) as an ancillary "molecular clip" ligand, have been synthesized and characterized. These ruthenium(II) complexes of bis(o-xylene)bipyridine-glycoluril self-associate in water through specific molecular recognition processes to form polycationic arrays. These arrays containing electrostatic binders as well as intercalator ligands at micromolar doses rapidly condense free DNA into globular nanoparticles of various sizes. The DNA condensation induced by these complexes has been investigated by electrophoretic mobility assay, dynamic light scattering, and transmission electron microscopy. The cellular uptake of complex-DNA condensates and the low cytotoxicity of these complexes satisfy the requirements of a gene vector. Carriers for DNA delivery: A series of mixed-ligand ruthenium(II) polypyridyl complexes of a bipyridine-functionalized molecular clip has been synthesized and characterized. These complexes rapidly condense free DNA into globular nanoparticles of various sizes. The DNA condensation induced by these complexes has been investigated by an electrophoretic mobility assay, dynamic light scattering, and transmission electron microscopy (see the respective images). The cellular uptake of complex-DNA condensates and the low cytotoxicity of these complexes satisfy the requirements of a gene vector.
机译：[Ru（bxbg）_2（NN）]〜（2+）类型的络合物，其中NN表示2,2'-联吡啶（bpy）（1），1,10-菲咯啉（phen）（2），双吡啶[ 3,2-d：2'，3-f]喹喔啉（dpq）（3）和双嘧啶[3,2-a：2'，3'-c]吩嗪（dppz）（4），并掺入bis（o -二甲苯）联吡啶-甘脲（bxbg）作为辅助的“分子夹”配体，已经合成并表征。这些双（邻二甲苯）联吡啶-甘脲的钌（II）配合物通过特定的分子识别过程在水中自缔合，形成聚阳离子阵列。这些包含微摩尔剂量的静电粘合剂和嵌入剂配体的阵列可将游离DNA快速凝结成各种大小的球形纳米颗粒。这些复合物诱导的DNA缩合已通过电泳迁移率测定，动态光散射和透射电子显微镜进行了研究。复合物-DNA冷凝物的细胞摄取以及这些复合物的低细胞毒性满足了基因载体的要求。 DNA传递的载体：合成并表征了联吡啶官能化分子夹子的一系列混合配体钌（II）聚吡啶基复合物。这些复合物迅速将游离DNA浓缩成各种大小的球形纳米颗粒。这些复合物诱导的DNA缩合已通过电泳迁移率测定，动态光散射和透射电子显微镜进行了研究（请参见相应的图像）。复合物-DNA冷凝物的细胞摄取以及这些复合物的低细胞毒性满足了基因载体的要求。

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