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首页> 外文期刊>Chemistry: A European journal >Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale
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Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale

机译：基于连续流反应器的磷酸化化合物的大规模酶促合成

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摘要

Acid phosphatase, an enzyme that is able to catalyze the transfer of a phosphate group from cheap pyrophosphate to alcoholic substrates, was covalently immobilized on polymethacrylate beads with an epoxy linker (Immobeads-150 or Sepabeads EC-EP). After immobilization 70 % of the activity was retained and the immobilized enzyme was stable for many months. With the immobilized enzyme we were able to produce and prepare D-glucose-6-phosphate, N-acetyl-D-glucosamine- 6-phosphate, allyl phosphate, dihydroxyacetone phosphate, glycerol-1-phosphate, and inosine-5'-monophosphate from the corresponding primary alcohol on gram scale using either a fed-batch reactor or a continuous-flow packed-bed reactor.
机译：酸性磷酸酶是一种能够催化磷酸酯基团从廉价的焦磷酸酯向乙醇底物转移的酶，已通过环氧连接剂（Immobeads-150或Sepabeads EC-EP）共价固定在聚甲基丙烯酸酯珠上。固定后，保留了70％的活性，固定的酶稳定了许多个月。借助固定化酶，我们能够生产和制备D-葡萄糖-6-磷酸酯，N-乙酰基-D-葡萄糖胺-6-磷酸酯，磷酸烯丙酯，磷酸二羟丙酮，甘油1-磷酸酯和肌苷5'-磷酸酯。使用补料分批反应器或连续流填充床反应器，以克为单位从相应的伯醇中提取。

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