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Facile Preparation of Core–Shell Magnetic Metal–Organic Framework Nanospheres for the Selective Enrichment of Endogenous Peptides

机译:核-壳磁性金属-有机骨架纳米球的简便制备,用于内源肽的选择性富集

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摘要

Facile preparation of core–shell magnetic metal– organic framework nanospheres by a layer-by-layer approach is presented. The nanospheres have high surface area (285.89 cm~2g~(-1)), large pore volume (0.18 cm~3g~(-1)), two kinds of mesopores (2.50 and 4.72 nm), excellent magnetic responsivity (55.65 emug~(-1)), structural stability, and good dispersibility. The combination of porosity, hydrophobicity, and uniform magnetism was exploited for effective enrichment of peptides with simultaneous exclusion of high molecular weight proteins. The nanospheres were successfully applied in the selective enrichment of endogenous peptides in human serum.
机译:提出了一种通过逐层方法轻松制备核-壳磁性金属-有机骨架纳米球的方法。纳米球具有高表面积(285.89 cm〜2g〜(-1)),大孔容(0.18 cm〜3g〜(-1)),两种中孔(2.50和4.72 nm),优异的磁响应性(55.65 emug) 〜(-1)),结构稳定性和良好的分散性。利用孔隙率,疏水性和均匀磁性的组合来有效富集肽,同时排除高分子量蛋白。纳米球已成功应用于人血清中内源肽的选择性富集。

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