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首页> 外文期刊>Chemistry: A European journal >DNA Dangling-End-Induced Colloidal Stabilization of Gold Nanoparticles for Colorimetric Single-Nucleotide Polymorphism Genotyping
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DNA Dangling-End-Induced Colloidal Stabilization of Gold Nanoparticles for Colorimetric Single-Nucleotide Polymorphism Genotyping

机译：比色法单核苷酸多态性基因分型的金纳米粒子的DNA悬空末端诱导胶体稳定。

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摘要

A single-nucleotide polymorphism (SNP) detection method was developed by combining single-base primer extension and salt-induced aggregation of gold nanoparticles densely functionalized with double-stranded DNA (dsDNA-AuNP). The dsDNA-AuNPs undergo rapid aggregation in a medium of high ionic strength, whereas particles having a single-base protrusion at the outermost surface disperse stably, allowing detection of a single-base difference in length by color changes. When SNP typing primers are used as analytes to hybridize to the single-stranded DNA on the AuNP surface, the resulting dsDNA-AuNP works as a visual indicator of single-base extension. A set of four extension reaction mixtures is prepared using each of ddNTPs and subsequently subjected to the aggregation assay. Three mixtures involving ddNTP that is not complementary to the SNP site in the target produce the aggregates that exhibit a purple color. In contrast, one mixture with the complementary ddNTP generates the single-base protrusion and appears red. This method could potentially be used in clinical diagnostics for personalized medicine.
机译：通过将单碱基引物延伸和盐诱导的金纳米颗粒（用双链DNA（dsDNA-AuNP）密集地功能化）相结合，开发了一种单核苷酸多态性（SNP）检测方法。 dsDNA-AuNP在高离子强度的介质中快速聚集，而在最外表面具有单碱基突出的颗粒稳定地分散，从而允许通过颜色变化来检测长度的单碱基差异。当使用SNP分型引物作为分析物与AuNP表面上的单链DNA杂交时，所得的dsDNA-AuNP可作为单碱基延伸的视觉指示。使用每种ddNTP制备一组四种延伸反应混合物，然后进行聚集测定。涉及ddNTP的三种混合物与目标中的SNP位点不互补，产生的聚集体呈紫色。相反，具有互补ddNTP的一种混合物会产生单碱基突出并显示为红色。该方法可潜在地用于个性化医学的临床诊断。

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