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首页> 外文期刊>Chemistry: A European journal >Rolling-Circle Amplification Detection of Thrombin Using Surface-Enhanced Raman Spectroscopy with Core-Shell Nanoparticle Probe
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Rolling-Circle Amplification Detection of Thrombin Using Surface-Enhanced Raman Spectroscopy with Core-Shell Nanoparticle Probe

机译：使用表面增强拉曼光谱和核-壳纳米粒子探针的凝血酶滚圆扩增检测

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摘要

An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased "hot-spot" was developed for the detection of thrombin. The sensor contains a SERS gold nanoparticle@Raman label@SiO2 core-shell nanoparticle probe in which the Raman reporter molecules are sandwiched between a gold nanoparticle core and a thin silica shell by a layer-by-layer method. Thrombin aptamer sequences were immobilized onto the magnetic beads (MBs) through hybridization with their complementary strand. In the presence of thrombin, the aptamer sequence was released; this allowed the remaining single-stranded DNA (ssDNA) to act as primer and initiate in situ RCA reaction to produce long ssDNAs. Then, a large number of SERS probes were attached on the long ssDNA templates, causing thousands of SERS probes to be involved in each biomolecular recognition event. This SERS method achieved the detection of thrombin in the range from 1.0 x 10(-12) to 1.0 x 10(-8) M and a detection limit of 4.2 x 10(-13) M, and showed good performance in real serum samples.
机译：为了检测凝血酶，开发了一种基于滚环放大（RCA）增加的“热点”的超灵敏表面增强拉曼光谱（SERS）传感器。该传感器包含一个SERS金纳米粒子@拉曼标记@ SiO 2核-壳纳米粒子探针，其中拉曼报告分子通过逐层方法夹在金纳米粒子核和二氧化硅薄壳之间。凝血酶适体序列通过与其互补链杂交而固定在磁珠（MBs）上。在凝血酶存在下，适体序列被释放。这使其余的单链DNA（ssDNA）充当引物并启动原位RCA反应以产生长的ssDNA。然后，将大量的SERS探针连接到长的ssDNA模板上，导致数千个SERS探针参与每个生物分子识别事件。该SERS方法实现了在1.0 x 10（-12）至1.0 x 10（-8）M范围内的凝血酶检测和4.2 x 10（-13）M的检测限，在真实血清样品中显示出良好的性能。

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