• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Light-Induced Porphyrin-Based Spectroscopic Ruler for Nanometer Distance Measurements
【24h】

Light-Induced Porphyrin-Based Spectroscopic Ruler for Nanometer Distance Measurements

机译：光诱导的基于卟啉的光谱尺用于纳米距离测量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We present a novel pulsed electron paramagnetic resonance (EPR) spectroscopic ruler to test the performance of a recently developed spin-labeling method based on the photoexcited triplet state (S=1). Four-pulse electron double resonance (PELDOR) experiments are carried out on a series of helical peptides, labeled at the N-terminal end with the porphyrin moiety, which can be excited to the triplet state, and with the nitroxide at various sequence positions, spanning distances in the range 1.8-8nm. The PELDOR traces provide accurate distance measurements for all the ruler series, showing deep envelope modulations at frequencies varying in a progressive way according to the increasing distance between the spin labels. The upper limit is evaluated and found to be around 8nm. The PELDOR-derived distances are in excellent agreement with theoretical predictions. We demonstrate that high sensitivity is acquired using the triplet state as a spin label by comparison with Cu(II)-porphyrin analogues. The new labeling approach has a high potential for measuring nanometer distances in more complex biological systems due to the properties of the porphyrin triplet state.
机译：我们提出了一种新颖的脉冲电子顺磁共振（EPR）光谱尺，以测试基于光激发三重态（S = 1）的最近开发的自旋标记方法的性能。在一系列螺旋肽上进行了四脉冲电子双共振（PELDOR）实验，在螺旋肽的N末端标记了卟啉部分，该卟啉部分可以被激发到三重态，并且在各个序列位置使用了氮氧化物，跨越距离在1.8-8nm之间。 PELDOR迹线可为所有标尺系列提供精确的距离测量值，显示出深层包络调制，其频率根据旋转标签之间距离的增加而逐渐变化。评估上限，发现约为8nm。 PELDOR得出的距离与理论预测非常吻合。我们证明通过使用三重态作为自旋标记与Cu（II）-卟啉类似物相比获得了高灵敏度。由于卟啉三重态的特性，这种新的标记方法具有在更复杂的生物系统中测量纳米距离的巨大潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号