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首页> 外文期刊>Acta Horticulturae >EST Sequencing and Analysis from Cold-Stored and Reconditioned Potato Tubers
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EST Sequencing and Analysis from Cold-Stored and Reconditioned Potato Tubers

机译：冷藏和翻新马铃薯块茎的EST序列分析

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摘要

Potato tubers under cold storage accumulate sugar, which causes browning of chips and French fries during frying. After cold storage, tubers often need to be treated under warm conditions to reduce the sugar content before frying. The gene expressionprofiles in these stored tubers are still largely unknown although there have been excellent reports on the molecular and physiological characteristics of cold-stored tubers. In this study, we sequenced and analyzed expressed sequence tags (ESTs) of potato tubers under different temperatures, as part of the Canadian Potato Genome Project (www.cpgp.ca). Greenhouse-grown tubers of 'Shepody' were used to establish cDNA libraries for EST sequencing. The cold-stored-tuber EST library (4°C, 3 months) was a normalized library, and the reconditioned-tuber EST library (4°C for 3 months, then reconditioned at 21°C for 3 days) was a standard library. ESTs were processed, clustered and assembled into high quality contiguous and unique sequences. Approximately 5,000 high quality ESTs were generated from each library. These ESTs and unigenes provide useful resources for studying the metabolic regulation and gene discovery in stored potato tubers.
机译：冷藏下的马铃薯块茎会积聚糖分，这会导致炸薯条和炸薯条变褐。冷藏后，通常需要在温暖的条件下对块茎进行处理，以减少油炸前的糖含量。尽管已经有关于冷储块茎的分子和生理学特征的极好的报道，但是在这些储藏的块茎中的基因表达谱仍然很大程度上未知。在这项研究中，我们对不同温度下马铃薯块茎的表达序列标签（EST）进行了测序和分析，这是加拿大马铃薯基因组计划（www.cpgp.ca）的一部分。使用温室栽培的“ Shepody”块茎建立用于EST测序的cDNA文库。冷藏的块茎EST文库（4°C，3个月）是归一化文库，翻新的块茎EST文库（4°C 3个月，然后在21°C翻新3天）是标准文库。 EST被处理，聚类并组装成高质量的连续且独特的序列。每个库产生了大约5,000个高质量的EST。这些EST和单基因为研究马铃薯块茎的代谢调控和基因发现提供了有用的资源。

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