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首页> 外文期刊>Acta Horticulturae >Metabolomics: Applications in Functional Biodiversity Analysis in Potato
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Metabolomics: Applications in Functional Biodiversity Analysis in Potato

机译:代谢组学:在马铃薯功能性生物多样性分析中的应用

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Transcriptomics, proteomics and metabolomics are increasingly used for broad-scale comparative analysis in the life sciences, forming important components of systems approaches to biological problems. This paper provides contemporary examples of the application of metabolite profiling and fingerprinting techniques to potato biology drawing on research carried out at the Scottish Crop Research Institute (SCRI). With regard to biodiversity, data are presented to show that specific taxonomic groups canbe separated both genetically and phytochemically using 73 accessions of wild Solatium species from the Common Potato Collection and that in adapted dipioid species such as Solanum phureja, metabolite profiling can be used to generate hypotheses on the processes that differentiate phureja in terms of flavour. Relevant to the establishment of quality traits of commercial relevance are temporal changes in the metabolome, thus profiling is being used to develop an understanding of changes associated with key stages in the tuber life cycle (tuberisation, tuber development and maturation, storage and sprouting). Finally, SCRI is using metabolomics as a comparative analysis tool to assess "natural" phytochemical variation to provide an indication of the impact of geographical location, crop management (e.g., high input, organic) and breeding systems (e.g., conventional, genetically modified [GM]) on metabolite profiles. This will provide important information on, for example, the potential for unintended compositional changes in GM potato and the extent to which such changes fall within the background of conventional breeding and production systems. Such data are important to policymakers, risk assessors, regulators and consumers
机译:转录组学,蛋白质组学和代谢组学被越来越多地用于生命科学的大规模比较分析,形成了生物学问题系统方法的重要组成部分。本文利用苏格兰作物研究所(SCRI)进行的研究,提供了代谢物谱分析和指纹技术在马铃薯生物学中的应用的当代实例。关于生物多样性,提供的数据表明,可以使用普通马铃薯收藏中的73种野生茄属物种在遗传和植物化学上分离特定的生物分类群,而在适应性二叶类物种(例如茄金盏花)中,可以使用代谢物谱来产生关于在风味方面区分金盏花的过程的假设。与商业相关的质量特征的建立有关的是代谢组的时间变化,因此,通过概况分析可了解与块茎生命周期(块茎化,块茎发育和成熟,贮藏和发芽)关键阶段有关的变化。最后,SCRI正在使用代谢组学作为比较分析工具来评估“自然”植物化学变异,以提供地理位置,作物管理(例如高投入,有机)和育种系统(例如常规的,基因改造的[ GM])。这将提供重要的信息,例如,关于转基因马铃薯意外成分变化的可能性以及此类变化在常规育种和生产系统背景下的程度。这些数据对于政策制定者,风险评估者,监管者和消费者而言非常重要。

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