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首页> 外文期刊>Acta Horticulturae >Liquid Culture System for Scutellaria Species
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Liquid Culture System for Scutellaria Species

机译：黄cut菌种液体培养系统

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摘要

In vitro liquid culture of Scutellaria species (Scutellaria lateriflora, S. costaricana and S. baicalensis) was investigated in different conditions. MS medium (Murashige and Skoog, 1962) (4.33 g L~(-1)) supplemented with BA (5 mu M), NAA (0.025 uM),sucrose (30 g L~(-1)) was used for two experiments with addition of agar (5 g L~(-1)) for agar treatments. Agar, liquid stationary (LS), liquid agitated (LA) and liquid culture with floating paper (FP) were compared for fresh weight (FW), dry weight (DW), % dry weight (%DW), multiplication ratio (MR) along with sucrose, media and water use. S. lateriflora, S. costaricana, S. baicalensis were cultured for 4, 6, 8 weeks respectively. LA and LS plantlets were more hyperhydric and had less MR compared to agar and FP plantlets. Biomass was higher in liquid culture except S. baicalensis plantlets in LS. Since LA and LS gave higher biomass, our further research is focused on a polyester fiber matrix in liquid that may prevent hyperhydricity and provide greater biomass. Agar, LS and liquid culture systems with matrix where plantlets kept in the gaseous phase were compared for similar responses with addition of hyperhydricity (visual assessment). The polyester fiber covered with seed germination paper was thematrix. Agar, LS and fiber 30 had 30 ml initial media and, fiber 20 began with 20 ml and had additional 5 ml at week 4 and 6. Plantlets were harvested after 8 weeks. Fiber 20 plantlets had significantly higher % DW for all species. Agar, fiber 20 and fiber 30 plantlets were less hyperhydric and higher MR compared to LS plantlets. Matrix prevented hyperhydricity and provided greater % DW with relatively higher MR.
机译：在不同条件下研究了黄cut菌种（Scutellaria Lateriflora，S。costaricana和S. baicalensis）的体外液体培养。使用MS培养基（Murashige和Skoog，1962）（4.33 g L〜（-1））补充BA（5μM），NAA（0.025 uM），蔗糖（30 g L〜（-1））进行两个实验加入琼脂（5 g L〜（-1））用于琼脂处理。将琼脂，液体固定液（LS），液体搅拌液（LA）和带浮纸的液体培养液（FP）进行了鲜重（FW），干重（DW），干重％（％DW），倍增比（MR）的比较以及蔗糖，培养基和水的使用。分别将S. Lateriflora，S。costaricana和S. baicalensis培养4、6、8周。与琼脂和FP幼苗相比，LA和LS幼苗的水合性更高，MR更低。在液体培养中生物量较高，除了在LS中的黄ba（S。baicalensis）苗。由于LA和LS提供了更高的生物量，因此我们的进一步研究集中在液体中的聚酯纤维基质上，该基质可以防止高水合度并提供更大的生物量。将琼脂，LS和具有基质的液体培养系统（其中小苗保留在气相中）进行比较，并增加了高水度（肉眼评估），以得到相似的响应。用种子发芽纸覆盖的聚酯纤维是基质。琼脂，LS和纤维30的初始培养基为30 ml，纤维20的起始培养基为20 ml，在第4和6周时再添加5ml。8周后收获小植株。纤维20苗的所有物种的DW百分比均显着较高。与LS幼苗相比，琼脂，20号纤维和30号纤维的幼苗的高羟基含量较低，MR较高。基质防止了高水合，并提供了较高的DW和较高的MR。

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