...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Horticulturae >Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic.
【24h】

Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic.

机译：在捷克共和国的苹果树和梨树栽培品种收集和生产人工林中的植物浆原体的分子鉴定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During the early summer and autumn of 2005, surveys were conducted in apple and pear production plantations and cultivar collection in the Research and Breeding Institute of Pomology, Holovousy (East Bohemia, Czech Republic). A total of 14 pear and 14 apple trees with symptoms of branch proliferation, dwarfism and premature leaf reddening, as well as asymptomatic trees, were studied for the presence of phytoplasmas. Nested PCR procedures with different sets of primers were used to amplify phytoplasma 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Among the plants evaluated, 7 pear and 7 apple trees were positive in nested PCR. Phytoplasmas belonging to apple proliferation group, subgroups 16Sr X-C and 16Sr X-A, were identified in 6 pear and 3 apple trees, respectively. RFLP analyses of phytoplasma 16Sr DNAs (R16F2/R16R2 nested PCR products) from 2 apple trees and one pear tree after digestion with endonuclease MseI showed a high level of similarity to the profile of phytoplasmas belonging to 16SrI-C subgroup. However, the fourth band (208 bp) was missing in the case of apple and a third band (235 bp) was not present in the profile from pear tree. One apple tree showed mixed infection with a phytoplasma belonging to 16SrX and 16SrI subgroup, and another apple tree exhibited mixed infection with a phytoplasma belonging to 16SrX and 16SrI-B subgroups. Fourteen trees (7 pear trees and 7 apple trees) were negative for phytoplasma. Experiments involving phytoplasma DNA sequencing from selected samples and its comparison with data available the GenBank confirmed the presence of phytoplasma under the 16Sr X-A, 16Sr X-C and 16Sr I-C subgroups, the type strains of which were apple proliferation, pear decline and clover phyllody phytoplasmas, respectively.
机译：在2005年初夏和秋季，在Holovousy绒球研究与育种研究所（捷克共和国波西米亚）对苹果和梨生产人工林和栽培品种的收集进行了调查。总共研究了14株梨和14棵苹果树，它们具有分支增生，矮化和叶过早变红的症状，以及无症状的植物是否存在植原体。使用具有不同引物组的巢式PCR程序扩增植物质体16SrDNA加上间隔区。 PCR产物的身份通过RFLP分析确认。在所评估的植物中，有7株梨和7株苹果树在巢式PCR中呈阳性。分别在6棵梨树和3棵苹果树中鉴定了属于苹果增生组的亚群16Sr X-C和16Sr X-A亚种。用内切核酸酶MseI消化后，对2棵苹果树和1棵梨树的植物质体16Sr DNA（R16F2 / R16R2巢式PCR产物）进行RFLP分析，显示与属于16SrI-C亚组的植物质体的特征高度相似。但是，在苹果的情况下，缺少第四条带（208 bp），而梨树的轮廓中没有第三条带（235 bp）。一棵苹果树显示出混合感染，其属于16SrX和16SrI子群的植物质体，另一棵苹果树显示出混合感染，其具有属于16SrX和16SrI-B子群的植物质体。 14棵树（7棵梨树和7棵苹果树）呈阴性。涉及从选定样品中进行植物质体DNA测序的实验以及与GenBank可用数据的比较，证实在16Sr XA，16Sr XC和16Sr IC亚组下分别存在植物质体，其类型菌株分别为苹果繁殖，梨衰退和三叶草叶状植物质体。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号