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首页> 外文期刊>Acta Horticulturae >Cloning ent-kaurene oxidase gene in chestnut and sequence analysis by semi-quantitative RT-PCR.
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Cloning ent-kaurene oxidase gene in chestnut and sequence analysis by semi-quantitative RT-PCR.

机译：克隆栗中的ent-kaurene氧化酶基因并通过半定量RT-PCR进行序列分析。

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摘要

In this study, a crucial gibberellins biosynthesis enzyme gene ent-kaurene oxidase was cloned from the library of chestnut using the strategy of homologous cloning. The length of the gene is 551 bp that is 75-84% homologous to other species. Semi-quantitative RT-PCR was used to investigate the expression pattern of ent-kaurene oxidase in mutant short catkin chestnut and the normal one. The result of Semi-quantitative RT-PCR showed that the signal detected in mutant short catkin chestnut was much stronger than that in the normal one. All results show that gibberellin ent-kaurene oxidase gene participates in the process of chestnut programmed cell death.
机译：在这项研究中，使用同源克隆策略从板栗文库中克隆了关键的赤霉素生物合成酶基因ent-kaurene氧化酶。该基因的长度为551 bp，与其他物种的同源性为75-84％。采用半定量RT-PCR方法研究了ent-kaurene氧化酶在突变矮柳絮栗和正常栗中的表达模式。半定量RT-PCR的结果表明，突变的短柔cat栗子中检测到的信号比正常人强得多。所有结果表明，赤霉素ent-kaurene氧化酶基因参与了板栗程序性细胞死亡的过程。

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