首页> 外文期刊>Acta Horticulturae >Diversity in Indian and some exotic onion cultivars as revealed by genomic and mitochondrial DNA.
【24h】

Diversity in Indian and some exotic onion cultivars as revealed by genomic and mitochondrial DNA.

机译:基因组和线粒体DNA揭示了印度和一些外来洋葱品种的多样性。

获取原文
获取原文并翻译 | 示例
           

摘要

Cytoplasmic genic male sterility in onion has been associated with the mitochondrial genome. Identification of male sterile lines and its maintainers is a major hurdle in exploitation of male sterility in onion hybrid seed production. In this study, we evaluated genomic and mitochondrial genome diversity to identify type of cytoplasm and male sterility among various onion varieties of Indian and exotic origin employing RAPD, SSR and non-radioactive RFLP molecular markers. Among the 68 used random decamer RAPD primers, 39 primers produced 445 total polymorphic bands with an average of 11.40 bands per primer. A total of 16 SSR primers were screened and 11 showed polymorphism and produced 86 polymorphic bands with an average of 8 polymorphic bands per primer. The RFLP studied using non-radioactive gene probes were cox1, cox2, atp6, atp9 and nad6. These studies demonstrate the diversity in the nuclear and mitochondrial genome of onion cultivars.
机译:洋葱中的细胞质遗传雄性不育与线粒体基因组有关。在洋葱杂种种子生产中,雄性不育系及其维持者的鉴定是利用雄性不育的主要障碍。在这项研究中,我们评估了基因组和线粒体的基因组多样性,以利用RAPD,SSR和非放射性RFLP分子标记物鉴定印度和外来起源的各种洋葱品种之间的细胞质类型和雄性不育。在68个使用过的随机decamer RAPD引物中,有39个引物产生了445个总多态性条带,每个引物平均具有11.40条带。总共筛选了16个SSR引物,其中11个表现出多态性,并产生86个多态性条带,每个引物平均有8个多态性条带。使用非放射性基因探针研究的RFLP是 cox1 , cox2 , atp6 , atp9 和 nad6 。这些研究表明洋葱品种的核和线粒体基因组中的多样性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号