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首页> 外文期刊>Acta Horticulturae >Bacterial canker of kiwifruit ( actinidia spp.): preliminary bacteriological studies in asymptomatic plant materials in northeastern Italy.
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Bacterial canker of kiwifruit ( actinidia spp.): preliminary bacteriological studies in asymptomatic plant materials in northeastern Italy.

机译：奇异果（ actinidia spp。）的细菌性溃疡病：意大利东北部无症状植物材料的初步细菌学研究。

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Italy is one of the largest producers of Kiwi [Actinidia deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson] and 'Hayward' is the most widespread cultivar. The development of reliable diagnostic methods for asymptomatic materials is crucial for the activity of Plant Protection Services in order to confirm the presence of the pathogen in the territory and in marketing materials. To detect the presence of Pseudomanas syringae pv. actidiniae (Psa), bacteria isolation from the surface and the columellae of asymptomatic fruits harvested in Lazio region with epidemics of bacterial canker was performed. For bacteria identification several biochemical tests and PCR analysis were carried out to discriminate Psa from the other kiwifruit pathogenic bacteria (i.e., Pseudomonas syringae pv. syringae and Pseudomonas viridiflava). The identification assays of the isolates obtained from the asymptomatic fruits of all samples excluded the presence of Psa. However, Psa lives in the phyllosphere together with non-pathogenic bacteria that may interfere in the diagnosis of the bacterial canker by means of false negative or positive results. Therefore, from leaves and shoots of asymptomatic plants of A. chinensis 'Hort16A' and A. deliciosa 'Hayward', collected from the Emilia Romagna region, epiphytic/endophytic bacteria were isolated and studied as possible causes of interference in the diagnostic protocol.
机译：意大利是猕猴桃（A. Chev。）C.F.的最大生产国之一。梁＆A.R.弗格森]和“海沃德”是最广泛使用的品种。开发无症状材料的可靠诊断方法对于植物保护服务的活动至关重要，以确认病原体在本地区和销售材料中的存在。要检测丁香假单胞菌 pv的存在。从拉齐奥地区收获的无症状果实表面进行细菌分离，并从细菌性溃疡病的流行中分离出 actidiniae （Psa）。为了鉴定细菌，进行了一些生化测试和PCR分析，以将Psa与其他猕猴桃致病菌（即丁香假单胞菌 pv。丁香假单胞菌和假单胞菌假单胞菌< / i>）。从所有样品的无症状果实获得的分离物的鉴定测定排除了Psa的存在。但是，Psa与非致病性细菌一起生活在叶球体内，它们可能通过假阴性或阳性结果而干扰细菌性溃疡病的诊断。因此，来自无症状植物A的叶和芽。 。从艾米利亚·罗马涅地区收集的deliciosa'Hayward'分离了附生/内生细菌，并研究了其可能干扰诊断方案。

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