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首页> 外文期刊>Acta Horticulturae >Study on genetic diversity of Aspergillus flavus population in Kerman province based on RAPD-PCR.
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Study on genetic diversity of Aspergillus flavus population in Kerman province based on RAPD-PCR.

机译：基于RAPD-PCR的克尔曼省黄曲霉种群遗传多样性研究。

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摘要

In this investigation, the population structure of A. flavus was analyzed using DNA fingerprinting based on RAPD-PCR by means of their primers including 54, 117 and 160. Total DNA was extracted from 54 monoconidial isolates that were isolated from contaminated seed and processing residue isolates samples and amplified according to specific PCR program. Each isolates was subjected to DNA fingerprinting and clonal lineages were determined. Phenetic analysis differentiated six distinct fingerprinting lineages between isolates. Clonal lineages A, B, D, C, E and F included 16.8, 53.7, 24, 1.8, 1.8 and 1.8% from tested isolates This study reveales that the population of the fungus did not show high level of diversity in Kerman province.
机译：在这项研究中，通过基于RAPD-PCR的引物（包括54、117和160），使用基于RAPD-PCR的DNA指纹分析了黄曲霉的种群结构。从54个单孢子分离物中提取了总DNA，这些分离物是从受污染的种子和加工残留物中分离得到的分离样品并根据特定的PCR程序进行扩增。对每个分离株进行DNA指纹图谱测定并确定克隆谱系。物候分析在分离株之间区分了六个不同的指纹谱系。克隆谱系A，B，D，C，E和F分别包含来自测试分离株的16.8％，53.7％，24％，1.8％，1.8％和1.8％这项研究表明，在克尔曼省，真菌种群没有表现出很高的多样性。

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