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首页> 外文期刊>Acta Horticulturae >Determination of the optimum microspore development stage and optimum culture medium in asparagus (Asparagus officinalis var. altilis L.) for anther culture.
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Determination of the optimum microspore development stage and optimum culture medium in asparagus (Asparagus officinalis var. altilis L.) for anther culture.

机译：确定用于花药培养的芦笋（Asparagus officinalis var。altilis L.）的最佳小孢子发育阶段和最佳培养基。

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摘要

Asparagus is a dioecious vegetable. The main target of asparagus breeders is to obtain hybrids composed of exclusively male plants, since they are more productive than female ones. Anther culture is a method to obtain haploids that can be doubled with colchicine treatment. In order to determine the optimal microspore development stage for androgenesis, anthers were stained with ethidium bromide and examined under fluorescence microscope. Anthers with microspores at the late uninucleate stage were cultured on Murashige and Skoog (MS) basal medium supplemented with three growth regulators at different concentrations (1 mg L-1 2.4 D+0.1 mg L-1 BA+0.5 mg L-1 NAA; 1 mg L-1 BA+5 mg L-1 NAA; 0.5 mg L-1 BA+1 mg L-1 NAA). The highest number of embryos (8.33%) and the number of plantlets (13.33%) were obtained on MS medium with 0.5 mg L-1 BA and 1 mg L-1 NAA.
机译：芦笋是一种雌雄异株的蔬菜。芦笋育种者的主要目标是获得仅由雄性植物组成的杂种，因为它们比雌性植物更有生产力。花药培养是获得可以通过秋水仙碱处理加倍的单倍体的方法。为了确定用于雄激素生成的最佳小孢子发育阶段，将花药用溴化乙锭染色并在荧光显微镜下检查。在补充了三种生长调节剂的Murashige和Skoog（MS）基础培养基上培养单核后期具有小孢子的花药（1 mg L -1 2.4 D + 0.1 mg L - 1 BA + 0.5 mg L -1 NAA; 1 mg L -1 BA + 5 mg L -1 NAA; 0.5毫克L -1 BA + 1毫克L -1 NAA）。在0.5 mg L -1 BA和1 mg L -1 的MS培养基上获得最高的胚数（8.33％）和幼苗数（13.33％）。 NAA。

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