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首页> 外文期刊>Acta Horticulturae >Influence of 6-benzylaminopurine and indole-3-butyric acid on in vitro propagation and secondary metabolites accumulation in Lamium album from lozen mountain.
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Influence of 6-benzylaminopurine and indole-3-butyric acid on in vitro propagation and secondary metabolites accumulation in Lamium album from lozen mountain.

机译：6-苄基氨基嘌呤和吲哚-3-丁酸对糖山紫m体外繁殖和次生代谢产物积累的影响。

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摘要

The effect of different concentrations (0.1-1.0 mg/L) of cytokinin BA (6-benzylaminopurine) and auxin IBA (indole-3-butyric acid) on the in vitro propagation and on the amount of secondary metabolites accumulation as well as their antioxidant capacity in the medicinal plant Lamium album was examined. A slight increase in shoot numbers was observed on Murashige and Skoog's (MS) medium supplemented with 0.8 mg/L BA when compared to control plants that were propagated on regular MS medium. All concentrations of BA suppressed root formation and stimulated callogenesis. Significant root formation on the MS medium supplemented with different concentrations of IBA was observed, especially at 0.7 mg/L IBA. Shoots cultivated on MS medium supplemented with BA from 0.6 up to 0.8 mg/L and IBA in concentration of 0.9 mg/L showed enhanced content of total phenols and flavonoids. The highest values of antioxidant activity were achieved on MS medium with 0.8 mg/L BA and 0.9 mg/L IBA. Compared to shoots and in vivo growing plants, significant stimulation of total phenolic content and antioxidant activity in forming callus and roots occurred on MS medium supplemented with 0.2, 0.5, 0.8 mg/L BA and 0.2, 0.5, 0.7 mg/L IBA. The amount of flavonoids in developed callus was twofold lower than the one observed in shoots. As far as we know, this is the first report to indicate stimulation activity of BA and IBA on the production and accumulation of phenolic compounds with high antioxidant capacity, especially in roots and callus, in in vitro propagated Lamium album.
机译：不同浓度（0.1-1.0 mg / L）的细胞分裂素BA（6-苄基氨基嘌呤）和生长素IBA（吲哚-3-丁酸）对体外繁殖，次级代谢产物累积量及其抗氧化剂的影响检查了药用植物Lamium Album的容量。与在常规MS培养基上繁殖的对照植物相比，在补充0.8 mg / L BA的Murashige和Skoog's（MS）培养基上观察到芽数略有增加。所有浓度的BA抑制根形成并刺激愈伤组织。在添加了不同浓度IBA的MS培养基上观察到明显的根形成，尤其是在0.7 mg / L IBA时。在补充有BA的0.6至0.8 mg / L的MS培养基和0.9 mg / L的IBA的MS培养基上培养的芽显示总酚和类黄酮的含量增加。在含有0.8 mg / L BA和0.9 mg / L IBA的MS培养基上，抗氧化剂活性最高。与苗和体内生长的植物相比，在添加0.2、0.5、0.8 mg / L BA和0.2、0.5、0.7 mg / L IBA的MS培养基上，总酚含量和形成愈伤组织和根的抗氧化活性得到了显着刺激。发达愈伤组织中黄酮的含量比芽中观察到的低两倍。据我们所知，这是第一个表明BA和IBA在体外繁殖的Lamium Albumin中对具有高抗氧化能力的酚类化合物（尤其是根和愈伤组织）产生和积累的刺激活性的报告。

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