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首页> 外文期刊>Acta Horticulturae >Clonal fidelity of chrysanthemum cultivars after long term micropropagation by stem segment culture.
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Clonal fidelity of chrysanthemum cultivars after long term micropropagation by stem segment culture.

机译：通过茎节培养进行长期微繁殖后的菊花品种的克隆保真度。

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摘要

Morphological characteristics of plants of chrysanthemum cultivars with daisy ('Reagan Sunny') and spider flower types ('Yellow Spider' and 'Cupper Spider') after long-term micropropagation (15 years) are investigated. Shoot cultures were established in December 1995 in nodal and internodal stem segment culture on Murashige and Skoog medium supplemented with alpha -naphthaleneacetic acid and benzylaminopurine (0.5 and 1.0 mg/L, respectively). Shoots are induced directly without callus phase, after four weeks. Multiplication of shoots by stem segment culture of all cultivars was achieved on same media composition through whole period of micropropagation. New shoots are formed by activation of axillary buds. Successful shoot rooting (100%) is achieved during end of May 2009 after three weeks of culture on Murashige and Skoog medium without plant regulators. Rooted chrysanthemum plantlets were planted at field conditions and successfully flowered during October. The same color, shape and size of flowers were confirmed for spider type cultivars in both regenerants and mother plants. Some variations in size, color and distribution of flower parts were observed in the daisy type chrysanthemum cultivar. The results from this study revealed clonally fidelity of spider type of chrysanthemum, which can be multiplied in long term cultures without any visible change in flower morphology. On the other hand, long term micropropagaton can be a used for good source for obtaining somaclonal variations for daisy type chrysanthemum.
机译：经过长期微繁殖（15年）后，研究了菊花（雏菊）（Reagan Sunny）和蜘蛛花类型（“ Yellow Spider”和“ Cupper Spider”）的植物形态学特征。 1995年12月，在补充α-萘乙酸和苄基氨基嘌呤（分别为0.5和1.0 mg / L）的Murashige和Skoog培养基上的节和节间茎节培养物中建立了芽培养物。四周后，直接诱导芽而没有愈伤组织阶段。在整个微繁殖期间，在相同的培养基组成下，所有品种的茎段培养均能繁殖出新芽。通过激活腋芽形成新芽。在没有植物调节剂的Murashige和Skoog培养基上培养三周后，在2009年5月下旬成功实现了生根（100％）。在田间条件下种植了菊花根小植株，并于10月成功开花。在再生植物和母本植物中，蜘蛛型栽培品种的花的颜色，形状和大小均相同。菊花型菊花品种在花的大小，颜色和分布上都有一定的变化。这项研究的结果表明，蜘蛛的菊花具有克隆保真度，可以在长期培养中繁殖，而花的形态没有明显变化。另一方面，长期的微促进素可以用作获得菊花型菊花体细胞变异的良好来源。

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