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Analysis of codon usage in Lilium longiflorum Thunb.

机译:百合中的密码子使用情况分析。

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摘要

The coding sequence of 123 selected proteins in Easter lily (Lilium longiflorum Thunb.) were analyzed using Codon W and CUSP programs. The frequency of codon usage that encoded amino acids was calculated and compared with dicotyledons and monocotyledons plants including soybean (Glycine max), cotton (Gossypium hirsutum), rice (Oryza sativa), wheat (Triticum aestivum). The results indicated that (1) the third position of codons in L. longiflorum was rich in G or C; (2) the content of G+C was equal to the A+U, it tends to use the codons ending with G or C; (3) six codons are determined as the optimal codons of L. longiflorum: CAU, CGU, ACC, GCC, GAG, GGC, which showed some difference from other monocots; (4) the codon usage frequency was obviously different from the dicotyledons such as soybean and cotton, but had a consistency in codon preference with the monocotyledons such as rice and wheat. We should apply these findings for L. longiflorum gene-transformation and selections of the high-efficient expression system.
机译:使用Codon W和CUSP程序分析了复活节百合(Lilium longiflorum Thunb。)中123种选定蛋白质的编码序列。计算编码氨基酸的密码子使用频率,并将其与双子叶植物和单子叶植物进行比较,包括大豆(Glycine max),棉花(Gossypium hirsutum),水稻(Oryza sativa),小麦(Triticum aestivum)。结果表明:(1)长寿花密码子的第三位富含G或C; (2)G + C含量等于A + U,倾向于使用以G或C结尾的密码子。 (3)确定了六个密码子作为长寿李的最佳密码子:CAU,CGU,ACC,GCC,GAG,GGC,与其他单子叶植物有一些差异; (4)密码子使用频率与大豆和棉花等双子叶植物明显不同,但与水稻和小麦等单子叶植物的密码子偏好保持一致。我们应该将这些发现应用于长寿菌基因转化和高效表达系统的选择。

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