...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Horticulturae >Anti-oxidant activity and non-genotoxic property of extracts of 'Longkong' Lansium domesticum fruits.
【24h】

Anti-oxidant activity and non-genotoxic property of extracts of 'Longkong' Lansium domesticum fruits.

机译：“龙岗” Lan果实提取物的抗氧化活性和非遗传毒性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lansium domesticum (Meliaceae) is an economic plant grown in the Southern, Eastern and Northern parts of Thailand. Fruit of L. domesticum (LD) is very popular in Thailand and commonly called 'Longkong'. The aim of this study was to investigate anti-oxidant activity and genotoxicity property of L. domesticum fruits, particularly the skins and seeds. The air-dried samples of skin (SK) and seed (SD) were extracted with 50 and 95% ethanol. The ethanolic extracts were partitioned between dichloromethane (DCM) and 50% aqueous ethanol. The aqueous phase was further extracted with ethyl acetate (EA) to yield a total of 12 fractions. Their anti-oxidant capacity was determined on superoxide anion (O2-.) scavenging by photochemiluminescence (PCL) assay, of both lipid (ACL) and water (ACW) soluble substance systems. The deoxyribose assay was also performed to assess their hydroxyl radicals (OH--.) scavenging activity. Our results suggested that skin of L. domesticum fruits possessed higher antioxidant potential than seeds, particularly when extracted with 50% ethanol and partitioned with ethyl acetate, which had high potential both hydrophilic and lipophilic antioxidant substances. The genotoxicity effect of ethanolic extract of L. domesticum was investigated in TK6 human lymphoblasts using cytokinesis-blocked micronucleus (CBMN) assay. Mitomycin C (MMC) served as a positive control and results were analyzed on micronucleus (MN) frequencies in binucleated cells (BNC). We found that there was no significant increase in MN frequencies in L. domesticum-treated cells whereas MMC-treated cells clearly exhibited a marked increase in MN frequency (40-72 MN/1000 BNC). It indicated that the ethanolic extracts of L. domesticum seeds were non-genotoxic to TK6 cells.
机译：Lansium domesticum（Meliaceae）是生长在泰国南部，东部和北部的经济植物。 L. domesticum（LD）的果实在泰国非常流行，通常被称为“龙岗”。这项研究的目的是调查家蝇的果实，特别是皮肤和种子的抗氧化活性和遗传毒性。用50％和95％的乙醇提取皮肤（SK）和种子（SD）的风干样品。将乙醇提取物在二氯甲烷（DCM）和50％乙醇水溶液之间分配。用乙酸乙酯（EA）进一步萃取水相，得到总共12个馏分。通过光化学发光法（PCL）清除脂质（ACL）和水（ACW）上的超氧阴离子（O 2 -）清除它们的抗氧化能力。物质系统。还进行了脱氧核糖测定以评估其羟基自由基（OH- -。）清除活性。我们的结果表明，L。domesticum果实的皮肤具有比种子更高的抗氧化潜力，尤其是当用50％乙醇提取并用乙酸乙酯分配时，其具有很高的亲水性和亲脂性抗氧化剂物质。使用胞质分裂阻滞微核（CBMN）分析了家蝇L.乙醇提取物在TK6人淋巴母细胞中的遗传毒性作用。丝裂霉素C（MMC）用作阳性对照，并对双核细胞（BNC）中的微核（MN）频率进行了分析。我们发现在L. domesticum处理的细胞中，MN频率没有显着增加，而MMC处理的细胞明显显示出MN频率显着增加（40-72 MN / 1000 BNC）。表明家蝇种子的乙醇提取物对TK6细胞无遗传毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号