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首页> 外文期刊>Acta Horticulturae >Effect of Phosphorus on In Vitro Growth and Development of Bromeliad Aechmea blanchetiana
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Effect of Phosphorus on In Vitro Growth and Development of Bromeliad Aechmea blanchetiana

机译:磷对凤梨Aechmea blanchetiana体外生长发育的影响

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The influence of different phosphorus concentrations in ionic balanced Murashige and SItoog medium on growth of Bromeliad Aechmea blanchetiana was studied. The plants of A. blanchetiana were obtained through in vitro sowing and were transferred to MSliquid medium (Murashige and Skoog, 1962), modified with 0.15, 0.30, 0.60, 1.25 or 2.50 mM P (as phosphate). Constant concentrations of all nutrients of MS medium were liept by ionic balance, prior to the adjustment of pH to 5.8. The results showed thatphosphorus influenced the growth of seedlings of A. blanchetiana cultured in MS medium, whereas the concentration of 2.5 mM of phosphate in modified MS medium provided the highest production of fresh and dry weight in both shoot and root system. Varianceanalysis of regression proved that the contents of variables nitrogen and potassium content in leaves of A. blanchetiana showed no significant trend with increasing phosphate concentration in modified MS medium. Phosphorus and sulfur content were significant only in the linear regression (a <0.05) and each mM phosphate added to MS medium led to an increase of 0.98 and 0.16 mg of phosphorus and sulfur in leaves, respectively.
机译:研究了离子平衡的Murashige和SItoog培养基中不同磷浓度对Bromeliad Aechmea blanchetiana生长的影响。通过体外播种获得了A. blanchetiana的植物,并将其转移至用0.15、0.30、0.60、1.25或2.50 mM P(磷酸盐)修饰的MSliquid培养基(Murashige和Skoog,1962)。在将pH调节至5.8之前,通过离子平衡限制了MS培养基中所有养分的恒定浓度。结果表明,磷会影响MS培养基中培养的A. blanchetiana的幼苗生长,而改良MS培养基中2.5 mM浓度的磷酸盐在茎和根系统中均能提供最高的鲜重和干重。回归的方差分析表明,在改良的MS培养基中,A。blanchetiana叶片中变量氮和钾的含量没有显示出随磷酸盐浓度增加的显着趋势。磷和硫的含量仅在线性回归中显着(a <0.05),向MS培养基中添加的每mM磷酸盐分别导致叶片中磷和硫的含量分别增加0.98和0.16 mg。

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