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首页> 外文期刊>ACS applied materials & interfaces >Regular Nanoscale Protein Patterns via Directed Adsorption through Self-Assembled DNA Origami Masks
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Regular Nanoscale Protein Patterns via Directed Adsorption through Self-Assembled DNA Origami Masks

机译：通过自组装的DNA折纸蒙版的直接吸附的常规纳米级蛋白质模式。

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DNA origami has become a widely used method for synthesizing well-defined nanostructures with promising applications in various areas of nanotechnology, biophysics, and medicine. Recently, the possibility to transfer the shape of single DNA origami nanostructures into different materials via molecular lithography approaches has received growing interest due to the great structural control provided by the DNA origami technique. Here, we use ordered monolayers of DNA origami nanostructures with internal cavities on mica surfaces as molecular lithography masks for the fabrication of regular protein patterns over large surface areas. Exposure of the masked sample surface to negatively charged proteins results in the directed adsorption of the proteins onto the exposed surface areas in the holes of the mask. By controlling the buffer and adsorption conditions, the protein coverage of the exposed areas can be varied from single proteins to densely packed monolayers. To demonstrate the versatility of this approach, regular nanopatterns of four different proteins are fabricated: the single-strand annealing proteins Red beta and Sak, the iron-storage protein ferritin, and the blood protein bovine serum albumin (BSA). We furthermore demonstrate the desorption of the DNA origami mask after directed protein adsorption, which may enable the fabrication of hierarchical patterns composed of different protein species. Because selectivity in adsorption is achieved by electrostatic interactions between the proteins and the exposed surface areas, this approach may enable also the large-scale patterning of other charged molecular species or even nanoparticles.
机译：DNA折纸已成为一种广泛使用的合成明确定义的纳米结构的方法，在纳米技术，生物物理学和医学的各个领域都具有广阔的应用前景。最近，由于DNA折纸技术提供的强大结构控制，通过分子光刻技术将单个DNA折纸纳米结构的形状转移到不同材料中的可能性受到了越来越多的关注。在这里，我们使用在云母表面具有内腔的DNA折纸纳米结构的有序单分子层作为分子光刻掩模，以在大表面积上制造规则的蛋白质图案。将被掩盖的样品表面暴露于带负电荷的蛋白质会导致蛋白质直接吸附到掩膜孔中暴露的表面区域。通过控制缓冲液和吸附条件，可以将暴露区域的蛋白质覆盖范围从单个蛋白质更改为紧密堆积的单分子层。为了证明这种方法的多功能性，我们制作了四种不同蛋白质的规则纳米图案：单链退火蛋白质Red Beta和Sak，铁存储蛋白质铁蛋白和血液蛋白质牛血清白蛋白（BSA）。我们进一步证明了定向的蛋白质吸附后DNA折纸蒙版的解吸，这可能使得能够制造由不同蛋白质种类组成的分层模式。由于吸附的选择性是通过蛋白质与暴露的表面积之间的静电相互作用实现的，因此该方法还可以实现其他带电分子种类甚至纳米粒子的大规模构图。

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