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首页> 外文期刊>ACS applied materials & interfaces >Rhipsalis (Cactaceae)-like Hierarchical Structure Based Microfluidic Chip for Highly Efficient Isolation of Rare Cancer Cells
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Rhipsalis (Cactaceae)-like Hierarchical Structure Based Microfluidic Chip for Highly Efficient Isolation of Rare Cancer Cells

机译：高效的分离稀有癌细胞的类脂类（仙人掌科）分层结构为基础的微流控芯片。

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摘要

The circulating tumor cells (CTCs), originating from the primary tumor, play a vital role in cancer diagnosis, prognosis, disease monitoring, and precise therapy. However, the CTCs are extremely rare in the peripheral bloodstream and hard to be isolated. To overcome current limitations associated with CTC capture and analysis, the strategy incorporating nanostructures with microfluidic devices receives wide attention. Here, we demonstrated a three-dimensional microfluidic device (Rm-chip) for capturing cancer cells with high efficiency by integrating a novel hierarchical structure, the "Rhipsalis (Cactaceae)"-like micropillar array, into the Rm-chip. The PDMS micropillar array was fabricated by soft-lithography and rapid prototyping method, which was then conformally plated with a thin gold layer through electroless plating. EpCAM antibody was modified onto the surface of the micropillars through the thiol-oligonucleotide linkers in order to release captured cancer cells by DNase I treatment. The antibody-functionalized device achieved an average capture efficiency of 88% in PBS and 83.7% in whole blood samples. We believe the Rm-chip provided a convenient, economical, and versatile approach for cell analysis with wide potential applications.
机译：起源于原发肿瘤的循环肿瘤细胞（CTC）在癌症诊断，预后，疾病监测和精确治疗中起着至关重要的作用。但是，四氯化碳在外周血中极为罕见，很难分离。为了克服当前与四氯化碳捕获和分析相关的局限性，将纳米结构与微流体装置结合在一起的策略受到了广泛关注。在这里，我们展示了一种三维微流控设备（Rm-chip），该设备通过将新型的分层结构“ Rhipsalis（Cactaceae）”样微柱阵列集成到Rm-chip中来高效捕获癌细胞。 PDMS微柱阵列通过软光刻和快速成型方法制造，然后通过化学镀敷在其上共形地镀上一层薄金层。为了通过DNase I处理释放捕获的癌细胞，将EpCAM抗体通过硫醇-寡核苷酸接头修饰到微柱的表面。抗体功能化装置在PBS中的平均捕获效率为88％，在全血样品中的平均捕获效率为83.7％。我们相信Rm芯片为细胞分析提供了一种方便，经济和通用的方法，具有广泛的潜在应用。

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