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首页> 外文期刊>ACS applied materials & interfaces >Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection
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Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

机译：可见光到近红外荧光石墨烯量子点上转换发射的增强，用于miRNA检测

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摘要

We developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp(2) carbon atoms of the GQD, mainly pi-pi stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through pi-pi stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.
机译：我们开发了一种用于检测特定microRNA（miRNA）序列的传感器，该传感器基于石墨烯量子点（GQD）和ssDNA-UCNP @ SiO2。拟议的传感器利用了GQD的sp（2）碳原子（主要是pi-pi堆积）与锚定在上转换纳米颗粒（UCNPs）上的DNA核碱基之间的相互作用。这种相互作用将GQD带到ssDNA-UCNP @ SiO2系统的表面，从而增强了上转换发射。另一方面，锚定在纳米颗粒上的单链DNA（ssDNA）链与其互补的miRNA序列的杂交会阻止UCNP通过pi-pi堆积与GQD相互作用的能力。结果，减少了荧光增强，这取决于miRNA序列的浓度。该效应用于创建检测限为10 fM的miRNA序列的传感器。

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