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首页> 外文期刊>American Journal of Physiology >Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat.
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Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat.

机译：内皮素ETB受体无效的大鼠的肌肉炎性炎症和Cajal间质细胞的丢失。

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摘要

Endothelin receptor null rats [ETB(-/-)] are a model for long-segment Hirschsprung's disease. These animals have significant intestinal distension (megaileum) proximal to a constricted region of the gastrointestinal tract lacking enteric ganglia. Experiments were performed to determine the pathophysiological changes that occur in these animals and to examine the tunica muscularis as a unique, immunologically active compartment. We observed abnormal intestinal flora in ETB(-/-) rats, which included a marked increase in gram-negative aerobes (Enterobacteriaceae) and anaerobes (Bacteroidaceae) in the distended region of the small intestine. Histochemical observations showed that neutrophilic infiltration was rarely or not observed, but the number of ED2 positive macrophages was increased in the tunica muscularis. Expression of IL-1beta and IL-6 mRNA was also significantly increased, and the level of CD14 (LPS receptors) were increased significantly in the tunica muscularis. Spontaneous phasic contractionswere irregular in the distended intestinal regions of ETB(-/-) rats, and this was associated with an increased number of macrophages and damage to interstitial cells of Cajal (ICC) as revealed by using Kit-like immunoreactivity and electron microscopy. These results suggest that ED2-positive resident macrophages may play an important role in the inflammation of tunica muscularis in ETB(-/-) rats. Increased numbers and activation of macrophages may result in damage to ICC networks leading to disordered intestinal rhythmicity in regions of the gut in which myenteric ganglia are intact.
机译：内皮素受体无效的大鼠[ETB（-/-）]是长节段性巨结肠疾病的模型。这些动物在缺乏肠神经节的胃肠道狭窄区域附近有明显的肠扩张（巨回肠）。进行实验以确定在这些动物中发生的病理生理学变化，并将肌膜检查为独特的具有免疫活性的腔室。我们在ETB（-/-）大鼠中观察到异常的肠道菌群，其中小肠扩张区的革兰氏阴性需氧菌（肠杆菌科）和厌氧菌（细菌科）明显增加。组织化学观察表明，很少或没有观察到嗜中性浸润，但是在肌膜中ED2阳性巨噬细胞的数量增加了。 IL-1beta和IL-6 mRNA的表达也显着增加，而肌膜中CD14（LPS受体）的水平也显着增加。自发相收缩在ETB（-/-）大鼠的扩张肠区域是不规则的，这与巨噬细胞数量的增加和对Cajal（ICC）的间质细胞的损害有关，如使用Kit-like免疫反应性和电子显微镜所揭示的。这些结果表明，ED2阳性的驻留巨噬细胞可能在ETB（-/-）大鼠的肌膜炎症中起重要作用。巨噬细胞数量的增加和活化可能会导致ICC网络受损，从而导致肠肌节完整的肠道区域的肠道节律紊乱。

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