• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Titin isoform-dependent effect of calcium on passive myocardial tension.
【24h】

Titin isoform-dependent effect of calcium on passive myocardial tension.

机译：钙对Titin亚型的依赖性对被动性心肌张力的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We studied the effects of Ca2+ on titin (connectin)-based passive tension in skinned myocardium expressing either predominantly N2B titin (rat right ventricle, RRV) or predominantly N2BA titin (bovine left atrium, BLA). Actomyosin-based tension was abolished to undetectably low levels by selectively removing the thin filaments with a Ca2+-insensitive gelsolin fragment (FX-45). Myocardium was stretched in the presence and absence of Ca2+, and passive tension was measured. Ca2+ significantly increased passive tension during and after stretch in the BLA. The increase was insensitive to the actomyosin inhibitor 2,3-butanedione 2-monoxime, supporting the conclusion that the effect is titin based. Passive tension did not respond to calcium in the RRV, indicating that passive tension developed by N2B titin is calcium insensitive. Western blot analysis and immunofluorescence studies indicated that N2BA titin expresses E-rich PEVK motifs, whereas they are absent from N2B titin, supporting earlier single molecule studies that reported that E-rich motifs are required for calcium sensitivity. We conclude that calcium affects passive myocardial tension in a titin isoform-dependent manner.
机译：我们研究了Ca2 +对主要表达N2B titin（大鼠右心室，RRV）或主要是N2BA titin（牛左心房，BLA）的皮肤心肌基于titin（connectin）的被动张力的影响。通过选择性除去带有Ca2 +不敏感凝溶胶蛋白片段（FX-45）的细丝，将基于肌动球蛋白的张力消除到不可检测的低水平。在存在和不存在Ca 2+的情况下拉伸心肌，并测量被动张力。在BLA拉伸期间和之后，Ca2 +显着增加了被动张力。该增加对放线菌酶抑制剂2，3-丁二酮2-单肟不敏感，支持结论是该作用是基于肌动蛋白的。被动张力对RRV中的钙无反应，表明N2B泰坦产生的被动张力对钙不敏感。蛋白质印迹分析和免疫荧光研究表明，N2BA titin表达富E的PEVK基序，而N2B titin却不存在，因此支持了较早的单分子研究，该研究报道，富E的基序是钙敏感性所必需的。我们得出结论，钙以肌动蛋白亚型依赖性方式影响被动型心肌张力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号