首页> 外文期刊>American Journal of Physiology >A mitochondrial role for catabolism of nitric oxide in cardiomyocytes not involving oxymyoglobin.
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A mitochondrial role for catabolism of nitric oxide in cardiomyocytes not involving oxymyoglobin.

机译:线粒体在不涉及氧肌红蛋白的心肌细胞中一氧化氮分解代谢中的作用。

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摘要

The maximal concentration of nitric oxide (NO) developing in cultured cells following stimulation of endogenous NO synthases was shown to be submicromolar by NO-selective microelectrode measurements. In electron paramagnetic resonance experiments with isolated and finely divided pericardium, NO was found to react with oxymyoglobin to form metmyoglobin provided that NO was supplied at concentrations in excess of a few micromolar. However, at NO concentrations achievable by endogenous sources, this reaction did not take place to any measurable extent. Oxidative conversion of NO to nitrite ion by cytochrome c oxidase appears to be the most plausible route for cellular catabolism of NO.
机译:内源性NO合酶刺激后,培养细胞中产生的一氧化氮(NO)的最大浓度通过NO-选择性微电极测量显示为亚微摩尔。在使用分离的且细碎的心包膜进行的电子顺磁共振实验中,发现只要提供的NO浓度超过几个微摩尔,NO就会与氧代肌红蛋白发生反应而形成肌红蛋白。但是,在内源可达到的NO浓度下,该反应未发生任何可测量的程度。细胞色素C氧化酶将NO氧化转化为亚硝酸根离子似乎是NO细胞分解代谢的最合理途径。

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