• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells.
【24h】

Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells.

机译：P2Y核苷酸受体和胞外核苷酸酶在静止和活化的大鼠肝星状细胞中的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Extracellular nucleotides regulate a variety of cellular activities, including proliferation of fibrogenic cells outside of the liver. However, the expression of receptors for extracellular nucleotides in hepatic stellate cells (HSC) is unknown. Thus our aims were to investigate the expression of mediators of nucleotide signaling in HSC and to determine whether extracellular nucleotides regulate HSC function. Confocal video microscopy was used to observe nucleotide-induced changes in cytosolic Ca(2+) (Ca(i)(2+)) in live HSC. P2Y receptor subtype expression and ectonucleotidase expression in quiescent and activated HSC were determined using RT-PCR, Northern blot, immunoblot, and confocal immunofluorescence. Functional ectonucleotidase activity was assessed using a colorimetric method. Nucleotide-sensitive procollagen-1 mRNA expression in activated HSC was assessed using real-time RT-PCR. Extracellular ATP increased Ca(i)(2+) in HSC; this was inhibited by the P2 receptor inhibitor suramin. Quiescent HSC expressed the P2Y subtypes P2Y(2) and P2Y(4) and were activated by ATP and UTP, whereas activated HSC expressed the P2Y subtype P2Y(6) and were activated by UDP and ATP. Activated but not quiescent HSC expressed the ectonucleotidase nucleoside triphosphate diphosphohydrolase 2, extracellular UDP tripled procollagen-1 mRNA expression in activated HSC, and this was inhibited by the P2Y receptor inhibitor suramin. HSC express functional P2Y receptors and switch the expression of P2Y receptor subtypes on activation. Moreover, HSC differentially regulate nucleoside triphosphate diphosphohydrolase expression after activation. Because activation of P2Y receptors in activated HSC regulates procollagen-1 transcription, P2Y receptors may be an attractive target to prevent or treat liver fibrosis.
机译：细胞外核苷酸调节多种细胞活性，包括肝外纤维化细胞的增殖。但是，尚不知道肝星状细胞（HSC）中胞外核苷酸受体的表达。因此，我们的目的是研究HSC中核苷酸信号传导介质的表达，并确定细胞外核苷酸是否调节HSC功能。共焦视频显微镜用于观察活HSC中核苷酸诱导的胞质Ca（2+）（Ca（i）（2+））的变化。使用RT-PCR，Northern印迹，免疫印迹和共聚焦免疫荧光法测定静止和激活的HSC中P2Y受体亚型的表达和胞外核苷酸酶的表达。使用比色法评估功能性外切核苷酸酶活性。使用实时RT-PCR评估活化的HSC中核苷酸敏感的procollagen-1 mRNA表达。细胞外ATP增加HSC中的Ca（i）（2+）;这被P2受体抑制剂苏拉明抑制。静态HSC表达P2Y亚型P2Y（2）和P2Y（4），并被ATP和UTP激活，而激活的HSC表达P2Y亚型P2Y（6），并被UDP和ATP激活。活化但不是静止的HSC表达了胞外核苷酸酶核苷三磷酸二磷酸水解酶2，细胞外UDP在活化的HSC中表达了三倍的procollagen-1 mRNA表达，并且被P2Y受体抑制剂苏拉明抑制。 HSC表达功能性P2Y受体，并在激活时切换P2Y受体亚型的表达。此外，HSC激活后差异调节核苷三磷酸二磷酸水解酶表达。因为激活的HSC中P2Y受体的激活调节了procollagen-1的转录，所以P2Y受体可能是预防或治疗肝纤维化的有吸引力的靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号