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首页> 外文期刊>American Journal of Physiology >Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis.
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Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis.

机译：液泡H（+）-ATPase的56 kDa B2亚基同工型在肾脏和附睾的质子分泌细胞中表达。

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摘要

B1 and B2 are two highly homologous isoforms of the vacuolar H(+)-ATPase (V-ATPase) 56-kDa B subunit. We investigated whether the B2 subunit is expressed alongside B1 in proton-secreting cells of the rodent kidney collecting duct (intercalated cells, IC) and epididymis (clear cells) by using antibodies against distinct COOH-terminal peptides from the two B isoforms. B2 was detected not only in the kidney proximal tubule, thick ascending limb, distal convoluted tubule, and connecting segment but also in A- and B-type IC of collecting ducts (CD) in both rat and mouse. B2 had a predominant cytoplasmic localization in most IC but was clearly located in a tighter apical band together with the V-ATPase 31-kDa E subunit in some A-IC, especially in the medulla. Apical membrane staining was confirmed by immunogold electron microscopy. B2 was very weakly expressed on the basolateral membranes of B-IC in control kidney CD, but some connecting segment B-IC had more distinct basolateral staining. In response to chronic carbonic anhydrase inhibition by acetazolamide, many A-IC showed a strong apical membrane localization of B2, where it colocalized with E and B1. In rat and mouse epididymis, B2 isoform expression was detected in clear cells, where it was concentrated in subapical vesicles. Unlike B1, B2 did not colocalize with the E subunit in the apical microvilli. These findings indicate that in addition to its role in the acidification of intracellular organelles, the B2 isoform could also contribute to transepithelial proton secretion and the maintenance of acid-base homeostasis.
机译：B1和B2是液泡H（+）-ATPase（V-ATPase）56-kDa B亚基的两个高度同源的同工型。我们通过使用针对来自两个B亚型的不同COOH末端肽段的抗体，研究了在啮齿动物肾脏收集管道的质子分泌细胞（插层细胞，IC）和附睾（透明细胞）中B2亚单位是否与B1一起表达。在大鼠和小鼠中，不仅在肾脏近端小管，粗大的上肢，远端回旋的小管和连接节段中检测到B2，而且在收集管（CD）的A型和B型IC中也检测到了B2。 B2在大多数IC中具有主要的胞质定位，但在某些A-IC中，尤其是在髓质中，与V-ATPase 31-kDa E亚基一起明显位于更紧密的根带中。顶端膜染色通过免疫金电子显微镜确认。 B2在对照肾CD的B-IC的基底外侧膜上非常弱地表达，但是一些连接节段B-IC具有更明显的基底外侧染色。响应于乙酰唑胺对慢性碳酸酐酶的抑制作用，许多A-IC均显示B2的根尖膜定位很强，与E和B1共定位。在大鼠和小鼠的附睾中，在透明细胞中检测到B2亚型表达，该细胞集中在根尖下囊泡中。与B1不同，B2在顶端微绒毛中未与E亚基共定位。这些发现表明，除了其在细胞内细胞器酸化中的作用外，B2亚型还可能有助于跨上皮质子的分泌和维持酸碱稳态。

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