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首页> 外文期刊>American Journal of Physiology >Influence of increased mechanical loading by hypergravity on the microtubule cytoskeleton and prostaglandin E2 release in primary osteoblasts.
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Influence of increased mechanical loading by hypergravity on the microtubule cytoskeleton and prostaglandin E2 release in primary osteoblasts.

机译：超重力增加的机械负荷对原代成骨细胞中微管细胞骨架和前列腺素E2释放的影响。

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摘要

Cells respond to a wide range of mechanical stimuli such as fluid shear and strain, although the contribution of gravity to cell structure and function is not understood. We hypothesized that bone-forming osteoblasts are sensitive to increased mechanical loading by hypergravity. A centrifuge suitable for cell culture was developed and validated, and then primary cultures of fetal rat calvarial osteoblasts at various stages of differentiation were mechanically loaded using hypergravity. We measured microtubule network morphology as well as release of the paracrine factor prostaglandin E2 (PGE2). In immature osteoblasts, a stimulus of 10x gravity (10 g) for 3 h increased PGE2 2.5-fold and decreased microtubule network height 1.12-fold without affecting cell viability. Hypergravity (3 h) caused dose-dependent (5-50 g) increases in PGE2 (5.3-fold at 50 g) and decreases (1.26-fold at 50 g) in microtubule network height. PGE2 release depended on duration but not orientation of the hypergravity load. As osteoblasts differentiated, sensitivity to hypergravity declined. We conclude that primary osteoblasts demonstrate dose- and duration-dependent sensitivity to gravitational loading, which appears to be blunted in mature osteoblasts.
机译：尽管重力对细胞结构和功能的贡献尚不清楚，但细胞对各种各样的机械刺激（例如流体剪切和应变）有反应。我们假设成骨的成骨细胞对超重力增加的机械负荷敏感。开发并验证了适用于细胞培养的离心机，然后使用超重力对处于分化各个阶段的胎鼠颅骨成骨细胞进行原代培养。我们测量了微管网络的形态以及旁分泌因子前列腺素E2（PGE2）的释放。在未成熟的成骨细胞中，以10倍重力（10 g）刺激3小时，可使PGE2增加2.5倍，并使微管网络高度降低1.12倍，而不会影响细胞活力。超重力（3 h）引起的PGE2剂量依赖性（5-50 g）增加（50 g为5.3倍），微管网络高度降低（50 g为1.26倍）。 PGE 2释放取决于持续时间，而不取决于超重力载荷的方向。随着成骨细胞的分化，对超重力的敏感性下降。我们得出结论，原代成骨细胞表现出对重力负荷的剂量和持续时间依赖性，这在成熟成骨细胞中似乎变得迟钝了。

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