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首页> 外文期刊>American Journal of Physiology >3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells.
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3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells.

机译：3D培养模型和计算机辅助视频显微镜分析非侵入性和侵入性支气管上皮细胞的迁移行为。

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To date, most of the studies in the field of cell migration have been applied to two-dimensional (2D) models. To mimic the three-dimensional (3D) conditions similar to those observed in vivo during tumor invasion, we developed a 3D model of cell migration in which cells were embedded in a collagen I matrix placed in a double-compartment chamber. Using time-lapse videomicroscopy and interactive cell tracking in a four-dimensional data set, we determined the cell trajectories and their migration kinetics. We compared the 2D and 3D migratory behavior of a noninvasive cell line (16HBE) with the migratory behavior of an invasive cell line (BZR). Our results show that the 3D migration kinetics of the noninvasive cell line were lower than the migration kinetics of the invasive cell line. In contrast, in 2D models, no significant difference was observed between the two cell lines. To validate our 3D model, we further investigated the effect of epidermal growth factor (EGF), a promoter of tumor cell motility and invasion on the noninvasive cell line (16HBE). EGF increased significantly the migration kinetics of the noninvasive cell line. Our results show that the 3D model of cell migration allowed us to differentiate the migratory behavior of invasive and noninvasive cells and that such a model can help in the development of molecular targeted therapy as it approaches the in vivo conditions.
机译：迄今为止，细胞迁移领域中的大多数研究已应用于二维（2D）模型。为了模拟类似于在肿瘤侵袭过程中在体内观察到的三维（3D）条件，我们开发了一种细胞迁移的3D模型，其中将细胞嵌入放置在双隔室中的I型胶原蛋白中。使用延时视频显微镜和交互式数据跟踪的多维数据集，我们确定了细胞轨迹及其迁移动力学。我们将无创细胞系（16HBE）的2D和3D迁移行为与有创细胞系（BZR）的迁移行为进行了比较。我们的结果表明，非侵入性细胞系的3D迁移动力学低于侵入性细胞系的3D迁移动力学。相反，在2D模型中，两个细胞系之间没有观察到显着差异。为了验证我们的3D模型，我们进一步研究了表皮生长因子（EGF），肿瘤细胞运动性和侵袭性的启动子对非侵袭性细胞系（16HBE）的影响。 EGF显着增加了非侵入性细胞系的迁移动力学。我们的结果表明，细胞迁移的3D模型使我们能够区分侵入性和非侵入性细胞的迁移行为，并且这种模型可以在接近体内条件时帮助开发分子靶向疗法。

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