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首页> 外文期刊>American Journal of Physiology >Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography.
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Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography.

机译：在高分辨率超声心动图的指导下，对小鼠心肌进行封闭胸腔注射。

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摘要

The mouse is an important model for the development of therapeutic stem cell/bone marrow cell implantation to treat ischemic myocardium. However, its small heart size hampers accurate implantation into the left ventricular (LV) wall. Precise injections have required surgical visualization of the heart, which is subject to complications and is impractical for delayed or repeated injections. Furthermore, the thickness of the myocardium is comparable to the length of a needle bevel, so surgical exposure does not prevent inadvertent injection into the LV cavity. We describe the use of high-resolution echocardiography to guide nonsurgical injections accurately into the mouse myocardial wall. We optimized this system by using a mixture of ultrasound contrast and fluorescent microspheres injected into the myocardium, which enabled us to interpret the ultrasound image of the needle during injection. Quantitative dye injection studies demonstrated that guided closed-chest injections and open-chest injections deliver comparable amounts of injectate to the myocardium. We successfully used this system in a mouse myocardial infarction model to target the injection of labeled cells to a region adjacent to the infarct. Intentional injection of tracer into the LV cavity resulted in a small accumulation in the myocardium, suggesting that non-guided cell injections into mouse hearts may appear to be successful even if the majority of the injectate is lost in the chamber. The use of this system will allow more precise cellular implantation into the mouse myocardium by accurately guiding injections to desired locations, confirming successful implantation of cells, in a clinically relevant time frame.
机译：小鼠是开发治疗性干细胞/骨髓细胞植入以治疗缺血性心肌的重要模型。但是，它的小心脏尺寸妨碍了向左心室（LV）壁的准确植入。精确注射需要对心脏进行手术可视化，这会引起并发症，并且对于延迟或重复注射是不切实际的。此外，心肌的厚度与针状斜面的长度相当，因此手术暴露不能防止无意中将其注射到LV腔中。我们描述了高分辨率超声心动图的使用，以指导非手术注射准确地进入小鼠心肌壁。我们通过将超声造影剂和注入心肌的荧光微球混合使用来优化该系统，这使我们能够在注射过程中解释针头的超声图像。定量染料注射研究表明，引导的闭胸注射和开胸注射可向心肌提供相当数量的注射。我们在小鼠心肌梗塞模型中成功使用了该系统，将标记细胞的注射靶向到与梗塞相邻的区域。有意将示踪剂注入左室，导致心肌中有少量积聚，这表明即使大部分注射液在腔室中丢失，向小鼠心脏的非引导性细胞注射也似乎是成功的。该系统的使用将通过在临床相关的时间框架内准确地将注射引导至所需位置，从而确认将细胞成功植入，从而将细胞更精确地植入小鼠心肌。

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