• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Imaging pancreatic beta-cells in the intact pancreas.
【24h】

Imaging pancreatic beta-cells in the intact pancreas.

机译：对完整胰腺中的胰岛β细胞进行成像。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have developed a method to visualize fluorescent protein-labeled beta-cells in the intact pancreas through combined reflection and confocal imaging. This method provides a 3-D view of the beta-cells in situ. Imaging of the pancreas from mouse insulin I promoter (MIP)-green (GFP) and red fluorescent protein (RFP) transgenic mice shows that islets, beta-cell clusters, and single beta-cells are not evenly distributed but are aligned along the large blood vessels. We also observe the solitary beta-cells in both fetal and adult mice and along the pancreatic and common bile ducts. We have imaged the developing endocrine cells in the embryos using neurogenin-3 (Ngn3)-GFP mice crossed with MIP-RFP mice. The dual-color-coded pancreas from embryos (E15.5) shows a large number of green Ngn3-expressing proendocrine cells with a smaller number of red beta-cells. The imaging technique that we have developed, coupled with the transgenic mice in which beta-cells and beta-cell progenitors are labeled with different fluorescent proteins, will be useful for studying pancreatic development and function in normal and disease states.
机译：我们已经开发出了一种通过组合反射和共聚焦成像在完整胰腺中可视化荧光蛋白标记的β细胞的方法。该方法提供了原位β细胞的3-D视图。小鼠胰岛素I启动子（MIP）-绿色（GFP）和红色荧光蛋白（RFP）转基因小鼠的胰腺成像显示，胰岛，β细胞簇和单个β细胞分布不均匀，但沿大的方向排列血管。我们还观察到在胎儿和成年小鼠以及胰腺和胆总管中的孤立的β细胞。我们已经成像了使用与MIP-RFP小鼠杂交的Neurogenin-3（Ngn3）-GFP小鼠在胚胎中发育的内分泌细胞。来自胚胎的双色编码胰腺（E15.5）显示大量表达Ngn3的绿色前内分泌细胞，以及少量红色β细胞。我们已经开发出的成像技术，再加上用不同的荧光蛋白标记了β细胞和β细胞祖细胞的转基因小鼠，对于研究正常和疾病状态下的胰腺发育和功能将很有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号