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首页> 外文期刊>American Journal of Physiology >IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass.
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IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass.

机译：条纹鲈鱼盐分驯化过程中IGF-I和分支IGF受体的表达和定位。

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摘要

The initial response of the IGF-I system and the expression and cellular localization of IGF type-I receptor (IGF-IR) were studied in the gill of a euryhaline teleost during salinity acclimation. Exposure of striped bass (Morone saxatilis) to hyperosmotic and hypoosmotic challenges induced small, transitory (<24 h) deflections in hydromineral balance. Transfer from freshwater (FW) to seawater (SW) induced an initial decrease in plasma IGF-I levels after 24 h in both fed and fasted fish. There was an overall decrease in liver IGF-I mRNA levels after SW transfer, suggesting that decreased plasma levels may be due to a decline in hepatic IGF-I synthesis. No changes were observed in gill IGF-I mRNA, but SW transfer induced an increase in gill IGF-IR mRNA after 24 h. Transfer from SW to FW induced an increase in plasma IGF-I levels in fasted fish. In fed fish, no significant changes were observed in either plasma IGF-I, liver, or gill IGF-I mRNA, or gill IGF-IR mRNA levels. In a separate experiment, FW-acclimated fish were injected with saline or IGF-I prior to a 24-h SW challenge. Rapid regain of osmotic balance following SW transfer was hindered by IGF-I. Immunohistochemistry revealed for the first time in teleosts that IGF-IR and Na(+)-K(+)-ATPase are localized in putative chloride cells at the base of the lamellae, identifying these cells in the gill as a target for IGF-I and IGF-II. Overall the data suggest a hyperosmoregulatory role of IGF-I in this species.
机译：在盐度驯化过程中，研究了鱼腥草硬骨鱼the的IGF-I系统的初始反应以及IGF-I受体（IGF-IR）的表达和细胞定位。条纹鲈鱼（Morone saxatilis）暴露于高渗和低渗的挑战下，导致矿物质平衡的短暂小偏差（<24小时）。从淡水（FW）转移到海水（SW）后，饲喂和禁食鱼的血浆中IGF-I含量均开始下降。 SW转移后肝脏IGF-I mRNA水平总体下降，提示血浆水平下降可能是由于肝脏IGF-I合成下降所致。在gIGF-1 mRNA中未观察到变化，但是SW转移在24小时后引起gIGF-1R mRNA增加。从SW到FW的转移导致禁食鱼血浆IGF-I水平升高。在饲喂鱼中，血浆IGF-1，肝脏或orIGF-1 mRNA或gIGF-1R mRNA水平均未观察到显着变化。在一个单独的实验中，在24小时的SW攻击之前，向FW适应的鱼注射盐水或IGF-I。 IGF-I阻碍了SW转移后渗透平衡的快速恢复。免疫组织化学首次在硬骨鱼中发现，IGF-1R和Na（+）-K（+）-ATPase位于薄片底部假定的氯化物细胞中，从而将ill中的这些细胞鉴定为IGF-I的靶标和IGF-II。总体而言，数据表明IGF-1在该物种中具有高渗透调节作用。

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