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首页> 外文期刊>American Journal of Physiology >Increased renal vascular reactivity to ANG II after unilateral nephrectomy in the rat involves 20-HETE.
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Increased renal vascular reactivity to ANG II after unilateral nephrectomy in the rat involves 20-HETE.

机译：大鼠单侧肾切除术后肾脏对ANG II的血管反应性增加涉及20-HETE。

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This study examined the role of intrarenal ANG II in the renal vascular reactivity changes occurring in the remaining kidney undergoing adaptation following contralateral nephrectomy. Renal blood flow responses to intrarenal injections of ANG II (0.25 to 5 ng) were measured in anesthetized euvolemic male Wistar rats 1, 4, 12, and 24 wk after uninephrectomy (UNX) or sham procedure (SHAM). At week 4, renal vasoconstriction induced by 2 ng ANG II was greater in UNX (69 +/- 5%) than in SHAM rats (50 +/- 3%; P < 0.01). This response was inhibited, by 50 and 66%, and by 20 and 25%, in SHAM and UNX rats, after combined injections of ANG II and losartan, or PD-123319 (P < 0.05), respectively. Characteristics of ANG II receptor binding in isolated preglomerular resistance vessels were similar in the two groups. After prostanoid inhibition with indomethacin, renal vasoconstriction was enhanced by 42 +/- 8% (P < 0.05), only in SHAM rats, whereas after 20-HETE inhibition with HET0016, it was reduced by 53 +/- 16% (P < 0.05), only in UNX rats. These differences vanished after concomitant prostanoid and 20-HETE inhibition in the two groups. After UNX, renal cortical protein expression of cytochrome P-450 2c23 isoform (CYP2c23) and cyclooxygenase-1 (COX-1) was unaltered, but it was decreased for CYP4a and increased for COX-2. In conclusion, renal vascular reactivity to ANG II was significantly increased in the postuninephrectomy adapted kidney, independently of protein expression, but presumably involving interactions between 20-HETE and COX in the renal microvasculature and changes in the paracrine activity of ANG II and 20-HETE.
机译：这项研究检查了肾内ANGII在对侧肾切除术后接受适应的其余肾脏中发生的肾血管反应性变化中的作用。在单肾切除术（UNX）或假手术（SHAM）后的麻醉的雄性维血性雄性Wistar大鼠1、4、12和24 wk中测量了对肾内注射ANG II（0.25至5 ng）的肾脏血流反应。在第4周，UNX（69 +/- 5％）中2 ng ANG II诱导的肾血管收缩大于SHAM大鼠（50 +/- 3％; P <0.01）。分别联合注射ANG II和氯沙坦或PD-123319后，在SHAM和UNX大鼠中，这种反应分别被抑制了50％和66％，分别被抑制了20％和25％（P <0.05）。两组中分离的肾小球前阻力血管中ANG II受体结合的特征相似。吲哚美辛抑制前列腺素类药物后，仅在SHAM大鼠中肾血管收缩增强了42 +/- 8％（P <0.05），而在HET0016抑制20-HETE后，肾血管收缩减少了53 +/- 16％（P < 0.05），仅适用于UNX大鼠。两组中前列腺素和20-HETE受到抑制后，这些差异消失了。 UNX后，细胞色素P-450 2c23亚型（CYP2c23）和环氧合酶1（COX-1）的肾皮质蛋白表达未改变，但CYP4a降低，COX-2升高。总之，在肾上腺切除术后适应的肾脏中，肾血管对ANG II的反应性显着增加，与蛋白表达无关，但可能涉及肾脏微脉管系统中20-HETE和COX的相互作用以及ANG II和20-HETE旁分泌活性的变化。 。

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